|
| Status |
Public on Sep 17, 2016 |
| Title |
RNA binding protein CPEB1 remodels host and viral RNA landscapes [RNA-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
In this study, we report that HCMV infection results in widespread alternative splicing (AS), shorter 3′-untranslated regions (3′UTRs) and polyA tail lengthening in host genes and CPEB1 depletion reverses infection-related post-transcriptional changes.
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| |
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| Overall design |
We performed RNA-seq for Mock (Non-targeting siRNA), human Cytomegalovirus (HCMV) with non-targeting siRNA, and CPEB1 siRNA treated human foreskin fibroblasts (HFFs). We also performed RNA-seq for lentivirus mediated GFP overexpression (OE) and CPEB1 overexpression human foreskin fibroblasts. Lastly, we performed TAIL-seq for Mock (Non-targeting siRNA), human Cytomegalovirus (HCMV) with non-targeting siRNA, and CPEB1 siRNA treated HFFs.
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| |
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| Contributor(s) |
Batra R, Yee B |
| Citation(s) |
27775709 |
| |
| Submission date |
Sep 09, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Gene Yeo |
| E-mail(s) |
geneyeo@ucsd.edu
|
| Organization name |
UCSD
|
| Street address |
2880 Torrey Pines Scenic Dr. Room 3805/Yeo Lab
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL15520 |
Illumina MiSeq (Homo sapiens) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (9)
|
| GSM2308766 |
Mock infected Non-targeting siRNA treated human foreskin fibroblasts [RNA-Seq] |
| GSM2308767 |
HCMV infected Non-targeting siRNA treated human foreskin fibroblasts [RNA-Seq] |
| GSM2308768 |
HCMV infected siCPEB1 siRNA treated human foreskin fibroblasts [RNA-Seq] |
| GSM2308769 |
Lentivirus mediated GFP overexpression in human foreskin fibroblasts [RNA-Seq] |
| GSM2308770 |
Lentivirus mediated CPEB1 overexpression in human foreskin fibroblasts-1 [RNA-Seq] |
| GSM2308771 |
Lentivirus mediated CPEB1 overexpression in human foreskin fibroblasts-2 [RNA-Seq] |
| GSM2308772 |
Mock infected Non-targeting siRNA treated human foreskin fibroblasts [Tail-Seq] |
| GSM2308773 |
HCMV infected Non-targeting siRNA treated human foreskin fibroblasts [Tail-Seq] |
| GSM2308774 |
HCMV infected siCPEB1 siRNA treated human foreskin fibroblasts [Tail-Seq] |
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| This SubSeries is part of SuperSeries: |
| GSE74250 |
Transcriptome analysis of diverse cell types infected with human cytomegalovirus |
|
| Relations |
| BioProject |
PRJNA342503 |
| SRA |
SRP087936 |
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