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| Status |
Public on Sep 08, 2017 |
| Title |
Expression profile of hematopoietic stem and progenitor cell (HSPC) compartment of FLT3-ITD and FLT3-ITD miR-155-/- mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The miR-155-dependent differences in gene expression in the HSPC compartment of FLT3-ITD mice is unknown. In this experiment, we performed RNA sequencing on FLT3-ITD and FLT3-ITD miR-155-/- mouse LKS cells.
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| Overall design |
RNA sequencing was performed on RNA extracted from Lin-, cKit+, Sca1+ cells isolated via flow cytometry from FLT3-ITD and FLT3-ITD miR-155-/- mice. 3 samples were submitted for sequencing for each experimental group. Each sample contains RNA from 3 mice, in order to get enough RNA from this rare stem cell population.
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| Contributor(s) |
O'Connell RM, Wallace JA |
| Citation(s) |
28432220 |
| |
| Submission date |
Sep 07, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Ryan O'Connell |
| E-mail(s) |
ryan.oconnell@path.utah.edu
|
| Phone |
801-213-4153
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| Organization name |
University of Utah
|
| Department |
Pathology
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| Lab |
EEJMRB 4280
|
| Street address |
15 N Medical Dr East
|
| City |
Salt lake city |
| State/province |
UT |
| ZIP/Postal code |
84112 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA342137 |
| SRA |
SRP087467 |
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