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Series GSE86480 Query DataSets for GSE86480
Status Public on Jul 31, 2017
Title Molecular signature for anastasis, recovery from the brink of apoptotic cell death
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Cells can survive effector caspase (caspase 3/7) activation in response to transient apoptotic stimuli, a process named anastasis. To characterize the molecular events that occur during anastasis, we performed whole transcriptome RNA sequencing of untreated, apoptotic, and recovering cells. We found that anastasis is an active, two-stage program with unique transcriptional profiles in each stage. We also identified 10 genes that specific to the early stage of anastasis.
Overall design 3hr ethanol treatment was used to induce apoptosis in Hela cells. Ethanol was washed away after 3hr treatment to allow cells to recover. Total RNA was prepared from mock-treated cells, ethanol-treated cells and cells after 1hr, 2hr, 3hr, 4hr, 8hr, 12hr recovery, followed by ribosomal RNA depletion. 3 biological replicates were included for each group. Sequencing was done using Ion Proton.
Contributor(s) Sun G, Guzman E, Zhou H, Kosik K, Montell DJ
Citation(s) 28768686
Submission date Sep 06, 2016
Last update date May 15, 2019
Contact name Kenneth S. Kosik
Organization name UCSB
Department NRI
Lab Kosik
Street address UC Santa Barbara
City Santa Barbara
State/province California
ZIP/Postal code 93106
Country USA
Platforms (1)
GPL17303 Ion Torrent Proton (Homo sapiens)
Samples (24)
GSM2304362 mock-treated cells replicate 1
GSM2304363 mock-treated cells replicate 2
GSM2304364 mock-treated cells replicate 3
BioProject PRJNA342034
SRA SRP086866

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GSE86480_genes.rpkm.gz 1.0 Mb (ftp)(http) RPKM
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