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| Status |
Public on Nov 27, 2018 |
| Title |
The Photorespiratory BOU Gene Mutation Alters Sulfur Assimilation and Its Crosstalk With Carbon and Nitrogen Metabolism in Arabidopsis thaliana |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
This study was aimed at elucidating the significance of photorespiratory serine (Ser) production for cysteine (Cys) biosynthesis. For this purpose, sulfur (S) metabolism and its crosstalk with nitrogen (N) and carbon (C) metabolism were analyzed in wildtype Arabidopsis and its photorespiratory bou-2 mutant with impaired glycine decarboxylase (GDC) activity. Foliar glycine and Ser contents were enhanced in the mutant at day and night. The high Ser levels in the mutant cannot be explained by transcript abundances of genes of the photorespiratory pathway or two alternative pathways of Ser biosynthesis. Despite enhanced foliar Ser, reduced GDC activity mediated a decline in sulfur flux into major sulfur pools in the mutant, as a result of deregulation of genes of sulfur reduction and assimilation. Still, foliar Cys and glutathione contents in the mutant were enhanced. The use of Cys for methionine and glucosinolates synthesis was reduced in the mutant. Reduced GDC activity in the mutant downregulated Calvin Cycle and nitrogen assimilation genes, upregulated key enzymes of glycolysis and the tricarboxylic acid (TCA) pathway and modified accumulation of sugars and TCA intermediates. Thus, photorespiratory Ser production can be replaced by other metabolic Ser sources, but this replacement deregulates the cross-talk between S, N, and C metabolism.
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| Overall design |
Arabidopsis thaliana plants [Wildtype Col-0 and a mutant line in Col-0 background, bou-2 (At5g46800) were grown in biological triplicates under high CO2 (3000 ppm) for 35 days, 12 hr light / 12 hr dark and then shifted to ambient CO2 24 hours before harvesting. Samples for RNASeq were taken two hours before ("end of night", EON) and two hours after ("beginning of day", BOD) the onset of light.
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| Contributor(s) |
Samuilov S, Brilhaus D, Flachbart S, Rademacher N, Arab L, Kuhnert F, Kopriva S, Weber AP, Altmann T, Rennenberg H |
| Citation(s) |
30559749 |
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Sladjana Samuilov, Dominik Brilhaus, Nadine Rademacher, Samantha Flachbart, Leila Arab, Saleh Alfarraj, Franziska Kuhnert, Stanislav Kopriva, Andreas P. M. Weber, Tabea Mettler-Altmann and Heinz Rennenberg. The Photorespiratory BOU Gene Mutation Alters Sulfur Assimilation and Its Crosstalk With Carbon and Nitrogen Metabolism in Arabidopsis thaliana. Front. Plant Sci. 2018. doi:10.3389/fpls.2018.01709
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| Submission date |
Sep 02, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Andreas PM Weber |
| E-mail(s) |
aweber@hhu.de
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| Organization name |
Heinrich-Heine-University
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| Department |
Institute for Plant Biochemistry
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| Street address |
Universitätsstr. 1
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| City |
Düsseldorf |
| ZIP/Postal code |
40225 |
| Country |
Germany |
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| Platforms (1) |
| GPL21179 |
Illumina HiSeq 3000 (Arabidopsis thaliana) |
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| Samples (12)
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| GSM2301212 |
Wildtype, beginning of day [1_WT_BOD_1] |
| GSM2301213 |
Wildtype, beginning of day [1_WT_BOD_2] |
| GSM2301214 |
Wildtype, beginning of day [1_WT_BOD_3] |
| GSM2301215 |
bou T-DNA insertion line, beginning of day [2_BOU_BOD_1] |
| GSM2301216 |
bou T-DNA insertion line, beginning of day [2_BOU_BOD_2] |
| GSM2301217 |
bou T-DNA insertion line, beginning of day [2_BOU_BOD_3] |
| GSM2301224 |
Wildtype, end of night [1_WT_EON_1] |
| GSM2301225 |
Wildtype, end of night [1_WT_EON_2] |
| GSM2301226 |
Wildtype, end of night [1_WT_EON_3] |
| GSM2301227 |
bou T-DNA insertion line, end of night [2_BOU_EON_1] |
| GSM2301228 |
bou T-DNA insertion line, end of night [2_BOU_EON_2] |
| GSM2301229 |
bou T-DNA insertion line, end of night [2_BOU_EON_3] |
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| Relations |
| BioProject |
PRJNA341576 |
| SRA |
SRP084243 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE86380_SupplementalDataset1.xlsx.gz |
16.5 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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