NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE86311 Query DataSets for GSE86311
Status Public on Aug 31, 2019
Title Transcriptomic analysis of swarm motility phenotype of Salmonella enterica serovar Typhimurium mutant defective in periplasmic glucan synthesis
Organisms Salmonella enterica subsp. enterica serovar Typhimurium; Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344
Experiment type Expression profiling by high throughput sequencing
Summary Movement of food-borne pathogens on moist surfaces enables them to migrate towards more favorable niches and facilitate their survival for extended periods of time. Salmonella enterica serovar Typhimurium mutants defective in OPG synthesis are unable to exhibit motility on moist surfaces (swarming) however their mobility in liquid (swim motility) remains unaffected. In order to understand the role of OPG in swarm motility, transcriptomic analysis was performed using cells growing on a moist agar surface.  In the opgGH-deletion mutant, lack of OPG significantly altered transcription of 1039 genes out of total 4712 genes (22%). Introduction of a plasmid borne copy of opgGH into the opgGH-deletion mutant restored normal expression of all but 30 genes, indicating a wide-range influence of OPG on gene expression under the swarm motility condition.  Major pathways that were differentially-expressed in opgGH mutants were motility, virulence and invasion, and genes related to the secondary messenger molecule, cyclic di-GMP.  These observations provide insights and help explain the pleiotropic nature of OPG mutants such as sub-optimal virulence and competitive organ colonization in mice, biofilm formation, and sensitivity towards detergent stress.  
 
Overall design The study comprised nine samples, three replicates for three strains of the organism.  It compared opgGH mutant Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344 with the wild type parental strain and the opgGH mutant carrying a plasmid borne wild-type copy of opgGH genes with the wild type parental strain.
 
Contributor(s) Bhagwat AA, Young L
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Aug 31, 2016
Last update date Aug 31, 2019
Contact name Arvind A Bhagwat
E-mail(s) arvind.bhagwat@ars.usda.gov
Phone 301-504-6443
Organization name United States Department of Agriculture
Department ARS, NEA, BARC, EMFSL
Lab Environmental Microbial and Food Safety Lab
Street address 10300 Baltimore Avenue
City Beltsville
State/province MD
ZIP/Postal code 20705
Country USA
 
Platforms (2)
GPL17070 Illumina HiSeq 2000 (Salmonella enterica subsp. enterica serovar Typhimurium)
GPL18823 Illumina HiSeq 2000 (Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344)
Samples (9)
GSM2299960 GH_243_4_TAGCTT_L005
GSM2299961 GH_243_5_GGCTAC_L005
GSM2299962 GH_243_6_CTTGTA_L005
Relations
BioProject PRJNA341384
SRA SRP083904

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE86311_aabhagwatprocessed.txt.gz 165.9 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap