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| Status |
Public on Jan 01, 2009 |
| Title |
Early responsive genes in human amnion epithelial FL cells induced by N-methyl-N’-nitro-N-nitrosoguanidine |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The alkylating agent N-methyl-N’-nitro-N-nitrosoguanidine (MNNG) induces cellular DNA damages and other comprehensive alterations that lead to chromosomal aberrations, mutations, tumor initiations, and cell death. However, the molecular mechanism of MNNG-induced cellular stress remains unclear.We have genome-wide analyzed early transcriptional responses of human FL amnion epithelial cells after exposure to three relatively low doses of MNNG (0.2, 1.0, and 10.0µM),and differential gene expression profiles were obtained 4 h after exposure using oligonucleotide microarrays followed by validation with quantitative real-time RT-PCR.
The results demonstrate that the MNNG-responsive genes are involved in multiple cellular biological processes including transcription regulation, signal transduction, cell cycle regulation, cytoskeleton organization, protein synthesis, immune response, metabolism, etc. The possible roles of these genes and their related pathways in MNNG-induced cellular responses were discussed. This study helps to draw the whole picture how cells respond to environmental chemical exposure via transcriptional regulation.
Keywords: Dose response
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| Overall design |
Human amnion epithelial FL cells were exposed to vehicle control (dimethyl sulfoxide) and increasing doses(0.2, 1.0, and 10.0µM)of N-methyl-N’-nitro-N-nitrosoguanidine(MNNG), respectively. The transcriptomes of the three treatments were compared to that of the control, respectively, to test the hypothesis that a characterized differential expression profile would be generated by exposure to various doses of this genotoxic agent.
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| Contributor(s) |
Li H, Shao J, Lu X, Gao Z, Yu Y |
| Citation(s) |
18644392 |
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| Submission date |
Jul 26, 2007 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Hongjuan Li |
| E-mail(s) |
lihj58@sina.com
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| Organization name |
Zhejiang University School of Medicine
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| Street address |
388 Yuhangtang Road
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| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310058 |
| Country |
China |
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| Platforms (2) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
| GPL97 |
[HG-U133B] Affymetrix Human Genome U133B Array |
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| Samples (16)
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| GSM213408 |
FL cells exposed to DMSO vehicle control, biological rep1 (HG-U133A) |
| GSM213409 |
FL cells exposed to DMSO vehicle control, biological rep2 (HG-U133A) |
| GSM213410 |
FL cells exposed to 0.2μM MNNG, biological rep1 (HG-U133A) |
| GSM213411 |
FL cells exposed to 0.2μM MNNG, biological rep2 (HG-U133A) |
| GSM213412 |
FL cells exposed to 1.0μM MNNG, biological rep1 (HG-U133A) |
| GSM213413 |
FL cells exposed to 1.0μM MNNG, biological rep2 (HG-U133A) |
| GSM213414 |
FL cells exposed to 10.0μM MNNG, biological rep1 (HG-U133A) |
| GSM213415 |
FL cells exposed to 10.0μM MNNG, biological rep2 (HG-U133A) |
| GSM213416 |
FL cells exposed to DMSO vehicle control, biological rep1 (HG-U133B) |
| GSM213417 |
FL cells exposed to DMSO vehicle control, biological rep2 (HG-U133B) |
| GSM213418 |
FL cells exposed to 0.2μM MNNG, biological rep1 (HG-U133B) |
| GSM213419 |
FL cells exposed to 0.2μM MNNG, biological rep2 (HG-U133B) |
| GSM213420 |
FL cells exposed to 1.0μM MNNG, biological rep1 (HG-U133B) |
| GSM213421 |
FL cells exposed to 1.0μM MNNG, biological rep2 (HG-U133B) |
| GSM213422 |
FL cells exposed to 10.0μM MNNG, biological rep1 (HG-U133B) |
| GSM213423 |
FL cells exposed to 10.0μM MNNG, biological rep2 (HG-U133B) |
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| Relations |
| BioProject |
PRJNA101767 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE8602_RAW.tar |
52.6 Mb |
(http)(custom) |
TAR (of CEL, EXP) |
| Processed data included within Sample table |
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