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| Status |
Public on Nov 08, 2016 |
| Title |
Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells [Bulk RNA-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Obtaining highly purified differentiated cells via directed differentiation from human pluripotent stem cells (hPSCs) is an essential step for the clinical application of hPSCs. Among the various conditions that should be optimized, the precise role and contribution of the extracellular matrix (ECM) during differentiation are relatively unclear. Here, using a short fragment of laminin 411 (LM411-E8), an ECM predominantly expressed in the vascular endothelial basement membrane, we demonstrated that the directed switching of defined ECMs robustly yields highly purified (>95%) endothelial progenitor cells (PSC-EPCs) from hPSCs in integrin-laminin axis and rho signaling pathway-dependent manner.
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| Overall design |
RNA-sequencing was conducted to identify the genes that were differentially expressed among differential conditions to induce the differentiation to the endothelial lineages.
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| Contributor(s) |
Ohta R, Niwa A, Saito M, Okada C, Watanabe A |
| Citation(s) |
27804979 |
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| Submission date |
Aug 18, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Akira Watanabe |
| E-mail(s) |
a.watanabe@cyberomix.com
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| Organization name |
CyberomiX Inc
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| Street address |
Rm504, Miyako Bldg, 233, Isa-cho, kamigyo-ku
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| City |
Kyoto |
| State/province |
Kyoto |
| ZIP/Postal code |
602-8407 |
| Country |
Japan |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (18)
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| GSM2283964 |
RNA-seq_KhES1_nc, rep1 |
| GSM2283965 |
RNA-seq_KhES1_nc, rep2 |
| GSM2283966 |
RNA-seq_KhES1_nc, rep3 |
| GSM2283967 |
RNA-seq_KhES1_411, rep1 |
| GSM2283968 |
RNA-seq_KhES1_411, rep2 |
| GSM2283969 |
RNA-seq_KhES1_411, rep3 |
| GSM2283970 |
RNA-seq_KhES1_411E8, rep1 |
| GSM2283971 |
RNA-seq_KhES1_411E8, rep2 |
| GSM2283972 |
RNA-seq_KhES1_411E8, rep3 |
| GSM2283973 |
RNA-seq_HUVEC, rep1 |
| GSM2283974 |
RNA-seq_HUVEC, rep2 |
| GSM2283975 |
RNA-seq_HUVEC, rep3 |
| GSM2283976 |
RNA-seq_HAEC, rep1 |
| GSM2283977 |
RNA-seq_HAEC, rep2 |
| GSM2283978 |
RNA-seq_HAEC, rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE85784 |
Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells |
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| Relations |
| BioProject |
PRJNA339382 |
| SRA |
SRP082319 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE85782_RAW.tar |
7.2 Mb |
(http)(custom) |
TAR (of TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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