|
| Status |
Public on Sep 25, 2017 |
| Title |
Thrombospondin1 replacement prevents cerebral cavernos malformations |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We have purified primary brain microvascular endothelial cells (BMEC) from mice bearing floxed alleles of Krit1 (Krit1fl/fl) and an endothelial specific tamoxifen-regulated Cre recombinase (Pdgfb-iCreERT2), and used these to delete Krit1 in a time-controlled manner (Krit1ECKO). To elucidate the pathogenesis of cerebral cavernous malformations (CCM) we used genome-wide RNA sequencing (RNA-seq) to characterize the transcriptome of primary BMEC following acute genetic inactivation of Krit1.
|
| |
|
| Overall design |
Treatment of Krit1f/f-Pdgfb-iCreERT2 BMEC with 5 µM-hydroxy-tamoxifen deleted Krit1 (Krit1ECKO) reduced KRIT1 mRNA and protein by >90% within 5 days compared to hydroxy-tamoxifen-treated Krit1fl/fl controls. Three independent biological replicates were used for the analysis.
|
| |
|
| Contributor(s) |
Lopez-Ramirez MA, Fonseca G, Ginsberg MH |
| Citation(s) |
28970240 |
| |
| Submission date |
Aug 15, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Miguel Alejandro Lopez-Ramirez |
| E-mail(s) |
malopezramirez@ucsd.edu
|
| Phone |
858-822-6487
|
| Organization name |
University of California, San Diego
|
| Department |
School of Medicine
|
| Street address |
9500 Gilman Drive
|
| City |
La Jolla |
| State/province |
California/San Diego |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA339007 |
| SRA |
SRP082179 |
Less...
More...