|
Status |
Public on Mar 01, 2017 |
Title |
Genome-wide analysis of histone modifications in Jurkat cells upon ELF-MF exposure |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
Summary |
To address whether ELF-MF influences the epigenetic landscape in the T cell lymphoma cell line Jurkat , we exposed Jurkat cells in the exponential growth phase to ELF MF or a sham control for 72 h, applying intermittently (5’ on/10’ off) a 50 Hz sine ELF-MF at a flux density of 1 mT. Additionally, cells were treated with the histone deacetylation inhibitor trichostatin A (TSA) at a subtoxic dose of 10 nM. Genome-wide profiling of active and repressive histone modifications did not reveal significant alterations. However, ELF-MF exposure has an influence on the robustness of the epigenetic landscape in leukaemic cells. Our data suggests that ELF-MF has a stochastic effect on the epigenetic landscape of individual cells.
|
|
|
Overall design |
H3K4me2 and H3K27me3 profiles from Jurkat cells before the exposure experiment (t0), ELF-MF and sham exposed for 72 h, and cells treated with the histone deacetylate inhibitor trichostatin A (10 nM TSA) analyzed by deep sequencing in douplicates of pools of three biological replicates each.
|
|
|
Contributor(s) |
Manser M, Sater M, Schmid C, Noreen F, Murbach M, Schurmann D, Schar P |
Citation(s) |
28266526 |
|
Submission date |
Aug 15, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Primo Schaer |
E-mail(s) |
primo.schaer@unibas.ch
|
Organization name |
University of Basel
|
Department |
Department of Biomedicine
|
Lab |
Genome Plasticity Laboratory
|
Street address |
Mattenstrasse 28
|
City |
Basel |
ZIP/Postal code |
4058 |
Country |
Switzerland |
|
|
Platforms (1) |
GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
Samples (16)
|
|
Relations |
BioProject |
PRJNA338926 |
SRA |
SRP082146 |