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Series GSE85455 Query DataSets for GSE85455
Status Public on Mar 21, 2017
Title ADAR1 controls apoptosis of stressed cells by inhibiting Staufen-mediated mRNA decay
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Both p150 and p110 isoforms of ADAR1 convert adenosine to inosine in double-stranded RNA (dsRNA). The p150 isoform suppresses the dsRNA sensing mechanism that activates the interferon induction mediated by the MDA5-MAVS signaling. In contrast, the biological function of the p110 isoform localized in the nucleus remains largely unknown. Here we show that stress-activated phosphorylation of ADAR1p110 by MKK6/p38 MAP kinases promotes its binding to Exportin-5 and nuclear export to the cytoplasm. Once translocated to the cytoplasmic, ADAR1p110 suppresses apoptosis of stressed cells by protecting many anti-apoptotic gene transcripts that contain 3’UTR dsRNA structures such as those consisting of inverted Alu repeats. ADAR1p110 competitively inhibits binding of Staufen1 to the 3’UTR dsRNAs and antagonizes the Staufen1-mediated mRNA decay mechanism. Our studies revealed a new stress response mechanism regulated by MAP kinases, in which ADAR1p110 translocates to the cytoplasm and regulates a class of mRNAs required for survival of stressed cells.
Overall design Examination of transcription changes due to ADAR1 and double ADAR1/STAU1 knockdown using RNA-seq
Contributor(s) Nishikura K, Sakurai M
Citation(s) 28436945
Submission date Aug 10, 2016
Last update date Sep 25, 2019
Contact name Priyankara J Wickramasinghe
Phone 2154956837
Organization name The Wistar Institute
Department Bioinformatics
Lab Genomics
Street address 3601 Spruce Street
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (6)
GSM2267301 siControl, replicate a
GSM2267302 siControl, replicate b
GSM2267303 siADAR1, replicate a
BioProject PRJNA338496
SRA SRP081249

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Supplementary file Size Download File type/resource
GSE85455_Nishikura_RSEM_NC.A.A_GEO.txt.gz 444.7 Kb (ftp)(http) TXT
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