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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 11, 2016 |
| Title |
Gene expression analysis of CD122HI versus CD122LO Vγ7+ murine small intestinal IEL at 14-17 days of age |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Background: The neonatal (D14-17) murine small intestinal epithelium is enriched for Vγ7+ IEL displaying an ‘immature’ CD122LO cell surface phenotype. We believe these cells are precursors for signature CD122HI Vγ7+ IEL that predominate among small intestinal IEL from postnatal D21.
Method: To further characterise this potential precursor-product relationship, CD122hi Vg7+ and CD122lo Vg7+ IEL were purified from individual D14-D17 mice on four independent occasions and compared by total RNAseq.
Conclusion: ~3000 genes are differentially expressed between CD122HI and CD122LO murine Vg7+ neonatal IEL
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| Overall design |
4 independent Paired samples of CD122hi Vg7+ and CD122lo Vg7+ IEL were purified from the small intestinal epithelium of pooled 14-17 day old mice. RNA was isolated from sorted samples. Gene expression analysis was performed by total RNAseq.
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| Contributor(s) |
Di Marco Barros R, Roberts N, Dart R, Vantourout P, Jandke A, Nussbaumer O, East P, Deban L, Cipolat S, Hart R, Iannitto ML, Laing A, Spencer-Dene B, Gibbons D, Irving P, Pereira P, Steinhoff U, Hayday A |
| Citation(s) |
27641500 |
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| Submission date |
Aug 10, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Rafael Di Marco Barros |
| E-mail(s) |
r.barros@ucl.ac.uk
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| Organization name |
Francis Crick Institute
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| Lab |
Immunosurveillance Lab, Lincolns Inn Fields Laboratory
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| Street address |
44 Lincolns Inn Fields
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| City |
London |
| ZIP/Postal code |
WC2A 3LY |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA338434 |
| SRA |
SRP081218 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE85422_RAW.tar |
10.4 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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