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| Status |
Public on Feb 10, 2017 |
| Title |
Wnt-responsive mesenchymal cells in dorsal back skin and ventral foot skin |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Canonical WNT-signaling is essential for placode formation irrespective of appendage type. At sites of placode initiation, Although WNT-signaling occurs in both epithelium and mesenchyme, the site of most intense activity as revealed by the WNT reporter Axin2-LacZ was in a zone just below the epithelial-mesenchymal interface. In ventral foot-skin, this WNT activity peaked at E17.5, concomitant with sweat bud fate commitment, while in dorsal back-skin, it began at E14.5, concomitant with HF fate specification.
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| Overall design |
To address whether WNT-signaling within this zone might regionally influence the transcriptional landscape of body-site mesenchymes to support distinct epithelial fates, we transcriptionally profiled the Axin2-positive and Axin2-negative dermal cells following their FACS-purification from E17.5 ventral foot-skin and E14.5 dorsal back-skin
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| |
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| Contributor(s) |
Lu CP, Fuchs E |
| Citation(s) |
28008008 |
| |
| Submission date |
Aug 05, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Catherine Pei-ju Lu |
| E-mail(s) |
clu@rockefeller.edu
|
| Organization name |
Rockefeller University
|
| Lab |
Fuchs Lab
|
| Street address |
1230 York Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA337944 |
| SRA |
SRP080997 |
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