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| Status |
Public on Mar 16, 2017 |
| Title |
Dido as a switchboard that regulates self-renewal and differentiation in embryonic stem cells (vsmutCT) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Transition from symmetric to asymmetric cell division requires precise coordination of differential gene expression. Embryonic stem cells (ESC) strongly express Dido3, whose C-terminal truncation impedes ESC differentiation while retaining self-renewal. We show that Dido3 binds to its gene locus via H3K4me3 and RNA pol II and, at differentiation onset, induces expression of its splice variant Dido1, which then leads to Dido3 degradation and downregulation of stemness genes. We propose that Dido isoforms act as a switchboard to regulate genetic programs for ESC transition from pluripotency maintenance to promotion of differentiation.
We used microarrays to determine how the deletion of exon 16 (C-terminal truncation) of the Dido3 affects gene expression that results in the suppression of ESC differentiation.
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| Overall design |
Mouse embryoid bodies from 2 conditions, wild type and truncated C-terminal Dido3 protein mutant overexpressing the missing terminal CT region (exon 16), were cultured in pararell and equal conditions. After 10 days development, RNA was extracted and processed for hybridization on Agilent microarrays (Agilent Mouse GE 4x44K v2 Microarray). Three biological replicates for each condition were processed simultaneously.
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| Contributor(s) |
Futterer A, De-Celis J |
| Citation(s) |
28330622 |
| |
| Submission date |
Aug 01, 2016 |
| Last update date |
Jan 19, 2018 |
| Contact name |
Carlos Martinez |
| Organization name |
Centro Nacional de Biotecnologia
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| Department |
Inmunology and Oncology
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| Lab |
411
|
| Street address |
C/Darwin 3
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| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28043 |
| Country |
Spain |
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| Platforms (1) |
| GPL10333 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version) |
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| Samples (3) |
| GSM2256418 |
10 days mouse embryoid body CT, biological rep1 |
| GSM2256419 |
10 days mouse embryoid body CT, biological rep2 |
| GSM2256420 |
10 days mouse embryoid body CT, biological rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE85029 |
Dido as a switchboard that regulates self-renewal and differentiation in embryonic stem cells |
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| Relations |
| BioProject |
PRJNA335994 |
