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| Status |
Public on Feb 01, 2017 |
| Title |
H3K36me2 occupancy profiling by high throughput sequencing from control and WHSC1 knockdown PC3 cells. |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
WHSC1 catalyzes dimethylation of lysine 36 on histone H3, which is profoundly upregulated in prostate cancer patients especially in metastatic PCa patients. We conduct ChIP sequencing in chromatin landscape induced by WHSC1 depleted in prostate cancer cell PC3 to understand the H3K36me2 genome-wide alterations.
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| Overall design |
Lentivirus-mediated RNA interference was used to knockdown WHSC1 expression in prostate cancer cell line PC3. The chromatin was prepared and followed by ChIP-Seq analysis by Active Motif, Inc. using the antibody against H3K36me2 (Millipore).
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| Contributor(s) |
Qin J, Li N |
| Citation missing |
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| Submission date |
Jul 26, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Ni Li |
| E-mail(s) |
lini@sibs.ac.cn
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| Organization name |
Institute of Health Sciences, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences
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| Lab |
Laboratory of Tumor Progression and Metastasis
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| Street address |
320 Yueyang Road, Shanghai, 200025 P.R. China
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| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA335399 |
| SRA |
SRP079919 |
