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Series GSE84869 Query DataSets for GSE84869
Status Public on Feb 01, 2017
Title H3K36me2 occupancy profiling by high throughput sequencing from control and WHSC1 knockdown PC3 cells.
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary WHSC1 catalyzes dimethylation of lysine 36 on histone H3, which is profoundly upregulated in prostate cancer patients especially in metastatic PCa patients. We conduct ChIP sequencing in chromatin landscape induced by WHSC1 depleted in prostate cancer cell PC3 to understand the H3K36me2 genome-wide alterations.
Overall design Lentivirus-mediated RNA interference was used to knockdown WHSC1 expression in prostate cancer cell line PC3. The chromatin was prepared and followed by ChIP-Seq analysis by Active Motif, Inc. using the antibody against H3K36me2 (Millipore).
Contributor(s) Qin J, Li N
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Submission date Jul 26, 2016
Last update date May 15, 2019
Contact name Ni Li
Organization name Institute of Health Sciences, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences
Lab Laboratory of Tumor Progression and Metastasis
Street address 320 Yueyang Road, Shanghai, 200025 P.R. China
City Shanghai
State/province Shanghai
ZIP/Postal code 200031
Country China
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (3)
GSM2252903 control H3K36me2_ChIPSeq
GSM2252904 shWHSC1 H3K36me2 ChIPSeq
GSM2252905 input DNA
BioProject PRJNA335399
SRA SRP079919

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Supplementary file Size Download File type/resource
GSE84869_RAW.tar 428.4 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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