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| Status |
Public on Mar 27, 2017 |
| Title |
Temporal-spatial Establishment of Initial Niche for the Primary Spermatogonial Stem Cell formation is Determined by an ARID4B Regulatory Network (ChIP-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The primary spermatogonial stem cells (SSCs), which arise from gonocytes during neonatal development, serve as a foundational self-renewing reservoir to ensure continuous production of spermatozoa throughout adulthood. The transformation of gonocytes into SSCs takes place in a niche established by Sertoli cells. To date, the factors that guide Sertoli cells to establish the initial stem cell niche remain largely unknown. Using Sertoli cell-specific Arid4b knockout (Arid4bSCKO) mice, we demonstrated that ablation of ARID4B resulted in failure to establish a niche for the SSC formation. We performed ChIP-Seq analysis to identify target genes of ARID4B in testes.
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| Overall design |
A pool of 108 testes was dissected from wild type male mice at P1.5 for ChIP, library preparation, sequencing, and initial data analysis. ChIP assays were performed using the anti-ARID4B antibody. Input DNA was used for control.
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| Contributor(s) |
Wu R, Wu M, Lanz RB |
| Citation(s) |
28207192 |
| |
| Submission date |
Jul 25, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Ray-Chang Wu |
| E-mail(s) |
rwu@gwu.edu
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| Phone |
2029941891
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| Organization name |
George Washington University
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| Department |
Biochemistry and Molecular Medicine
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| Street address |
2300 I St, NW
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| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20037 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE84808 |
Temporal-spatial Establishment of Initial Niche for the Primary Spermatogonial Stem Cell formation is Determined by an ARID4B Regulatory Network |
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| Relations |
| BioProject |
PRJNA331159 |
| SRA |
SRP079692 |
