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Series GSE84539 Query DataSets for GSE84539
Status Public on Jan 04, 2018
Title The whole transcriptome expression profiling comparison between the Pin1 knockIn in Hose cells and Pin1 knockdown in ovarian cancer MCAS and SK-OV-3 cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To discover the abnormal regulatory role of Pin1 in the ovarian cancer, The Pin1 full cDNA was knockIn (KI) the normal ovarian epithelium (Hose), and parallelly Pin1 was knockdown in the Ovarian cancer cells MCAS and sk-ov-3, and then compared the expression profiles of them to discovery the key features regulated by Pin1.
Overall design There are three groups cells used for this expression profile comparison : Hose Pin1 KnockIn (KI) and Hose scramble control; Skov3 Pin1 knockdown (KD) and Scramble control; Mcas Pin1 knockdown and scramble control. The RNA was extracted from well cultured cells using TRIzol reagent, and whole transcriptome gene expression profiling were performed using an Affymetrix Human Gene 1.1 ST whole transcriptome microarray platform.
Contributor(s) Liu S, Ng S
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Submission date Jul 18, 2016
Last update date Jan 05, 2018
Contact name Shu-Wing Ng
Phone 6172780072
Organization name Brigham and Women’s Hospital; Harvard Medical School
Department Department of Obstetrics, Gynecology and Reproductive Biology
Lab Laboratory of Gynecologic Oncology
Street address 221 Longwood Avenue
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL11532 [HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version]
Samples (6)
GSM2241689 Hose with scramble control KnockIn
GSM2241690 Hose with Pin1 KnockIn
GSM2241691 MCAS with Scramble Ctrl knockdown
BioProject PRJNA329557

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE84539_RAW.tar 25.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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