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| Status |
Public on Jan 04, 2018 |
| Title |
The whole transcriptome expression profiling comparison between the Pin1 knockIn in Hose cells and Pin1 knockdown in ovarian cancer MCAS and SK-OV-3 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To discover the abnormal regulatory role of Pin1 in the ovarian cancer, The Pin1 full cDNA was knockIn (KI) the normal ovarian epithelium (Hose), and parallelly Pin1 was knockdown in the Ovarian cancer cells MCAS and sk-ov-3, and then compared the expression profiles of them to discovery the key features regulated by Pin1.
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| Overall design |
There are three groups cells used for this expression profile comparison : Hose Pin1 KnockIn (KI) and Hose scramble control; Skov3 Pin1 knockdown (KD) and Scramble control; Mcas Pin1 knockdown and scramble control. The RNA was extracted from well cultured cells using TRIzol reagent, and whole transcriptome gene expression profiling were performed using an Affymetrix Human Gene 1.1 ST whole transcriptome microarray platform.
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| Contributor(s) |
Liu S, Ng S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 18, 2016 |
| Last update date |
Jan 05, 2018 |
| Contact name |
Shu-Wing Ng |
| E-mail(s) |
sng@partners.org
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| Phone |
6172780072
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| Organization name |
Brigham and Women’s Hospital; Harvard Medical School
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| Department |
Department of Obstetrics, Gynecology and Reproductive Biology
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| Lab |
Laboratory of Gynecologic Oncology
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| Street address |
221 Longwood Avenue
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL11532 |
[HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA329557 |
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