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| Status |
Public on Aug 15, 2016 |
| Title |
Gene expression data from yerba mate treated and non-treated cultured PBMCs activated with phytohemagglutinin |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Yerba mate (YM) has been shown to have anti-inflammatory properties in several studies. However, this effect has been found mainly in obesity-related in inflammation. The aim of this work was to study the effect of YM in cultured peripheral blood mononuclear cells to see whether it has anti-inflammatory properties. We stimulated peripheral blood mononuclear cells in vitro with phitohemaglutinin in the presence of yerba mate and determined their activation measuring the the expression of CD25 by flow cytometry. We observed that YM treatment produced a dose-dependent reduction in PBMC activation (CD25 positive cells) when they were stimulated with PHA. This effect was also observed in T cells (CD3 positive) subpopulation. Microarray analysis revealed the differential expression of 128 genes in YM-treated cells. According to a protein-protein interaction database, these genes were highly connected and they are involved in inflammatory response. In summary, it was demonstrated that YM produces a reduction in the amount of activated cells under the stimulation of PHA. Therefore, it might be used in diseases with an inflammatory component.
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| Overall design |
PBMC samples from a healthy individual were cultured and stimulated with phytohemagglutinin. Gene expression of yerba mate treated and non-treated cells was analyzed in duplicates, having in total four microarrays.
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| Contributor(s) |
Muñoz-Culla M, Sáenz-Cuesta M, Guereca-Barandiaran MJ, Ribeiro ML, Otaegui D |
| Citation(s) |
27714004 |
| |
| Submission date |
Jul 14, 2016 |
| Last update date |
Oct 29, 2018 |
| Contact name |
Maider Muñoz-Culla |
| E-mail(s) |
maider.munoz@ehu.eus
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| Organization name |
Biodonostia Institute
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| Street address |
Paseo Dr.Begiristain s/n
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| City |
San Sebastian |
| ZIP/Postal code |
20014 |
| Country |
Spain |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (4)
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| GSM2233146 |
cultured PBMCs_100 ug/ml YM treatment_rep2 |
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| Relations |
| BioProject |
PRJNA329094 |
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