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Series GSE84352 Query DataSets for GSE84352
Status Public on Aug 10, 2017
Title Read-through transcription as a general mechanism mediating methylation and silencing of intragenic CGIs [H3K36me3_ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The human genome contains approximately 27,700 CpG islands (CGIs). Most are associated with promoters and their DNA is nearly always unmethylated. By contrast, CGIs lying within the bodies of genes usually become methylated during differentiation and development. CGIs also normally become methylated at X-inactivated and imprinted genes and abnormally methylated in genome rearrangements and in malignancy. In such circumstances, methylation of CGIs is often associated with RNA transcripts reading through these elements but the relationship of this RNA to methylation of CGIs is not clear. Here we investigated a previously described form of α-thalassemia caused by a genome rearrangement leading to abnormal transcription and DNA methylation of the CGI at the promoter of the α-globin gene. We show that transcription per se is responsible for DNMT3B-mediated methylation of the globin CGI, and that this is a general mechanism responsible for methylation of most intragenic CpG islands.
Overall design One H3K36me3 ChIP-seq sample and its sonication input (no replicates)
Contributor(s) Jeziorska DM, Telenius J, Hughes J, Higgs DR, Tufarelli C
Citation(s) 28827334
Submission date Jul 13, 2016
Last update date Sep 13, 2019
Contact name Jim Hughes
Phone 1865222113
Organization name University of Oxford
Department MHU
Lab Genome Biology Group
Street address Weatherall Institute Of Molecular Me
City oxford
ZIP/Postal code OX3 9DS
Country United Kingdom
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (2)
GSM2232660 H3K36me3_ChIPseq
GSM2232661 sonication INPUT_ChIPseq
This SubSeries is part of SuperSeries:
GSE84355 Read-through transcription as a general mechanism mediating methylation and silencing of intragenic CGIs
BioProject PRJNA328979
SRA SRP078436

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Supplementary file Size Download File type/resource
GSE84352_RAW.tar 933.2 Mb (http)(custom) TAR (of BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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