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| Status |
Public on Jul 13, 2016 |
| Title |
Nutrigenomic effects of glucosinolates on liver, muscle and distal kidney of Atlantic salmon |
| Platform organisms |
Oncorhynchus mykiss; Salmo salar |
| Sample organism |
Salmo salar |
| Experiment type |
Expression profiling by array
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| Summary |
Glucosinolates (GLs) present in plants from the Brassica family hold promise in the biological control of the skin parasite salmon louse (Lepeophtheirus salmonis) in farmed Atlantic salmon. We recently reported significantly reduced lice infestation in fish fed GLs. However, their wider application requires better knowledge of GLs’ actions, including positive and adverse effects that may occur under the overexposure. Microarray analyses performed in the liver, muscle and distal kidney of salmon under high dose of GLs suggested massive tissue remodeling and reduction of cellular proliferation in skeletal muscle and liver. In the distal kidney, gene expression profiles induced under the high dose of GLs pointed to activation of anti-fibrotic responses. At the same time, prevalent activation of genes from the Phase-2 detoxification pathways could be considered part of beneficial effects. Multiple gene expression evidence suggested GLs-mediated iron/heme withdrawal response, including increase in heme degradation in muscle (up-regulation of heme oxygenase-1), decrease of its synthesis in liver (down-regulation of porphobilinogen deaminase) and increase in iron sequestration from blood (hepatic induction of hepcidin-1 and renal induction of intracellular storage protein ferritin). This could be beneficial upon encounter with the parasite, which depends on the provision of iron/heme by the host.
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| Overall design |
Atlantic salmon received control feed and feed with 13% of the GL-containing raw ingredient during three weeks. Microarray analyses were performed in distal kidney, liver and muscle.
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| Contributor(s) |
Krasnov A, Jodaa Holm H, Skugor S |
| Citation(s) |
27164990, 27955686 |
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| Submission date |
Jul 12, 2016 |
| Last update date |
Jul 12, 2019 |
| Contact name |
Aleksei Krasnov |
| E-mail(s) |
aleksei.krasnov@nofima.no
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| Phone |
+47 97602165
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| Organization name |
Nofima AS
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| Department |
Fish Health
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| Street address |
Osloveien 1
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| City |
Aas |
| ZIP/Postal code |
1430 |
| Country |
Norway |
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| Platforms (1) |
| GPL16555 |
Agilent-030860 Salmon Immunity and Quality 15k custom array_SIQ6 |
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| Samples (30)
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| GSM2230841 |
distal kidney, control feed, fish 31 |
| GSM2230842 |
distal kidney, control feed, fish 36 |
| GSM2230843 |
distal kidney, feed with glucosinolates, fish 12 |
| GSM2230844 |
distal kidney, feed with glucosinolates, fish 14 |
| GSM2230845 |
distal kidney, feed with glucosinolates, fish 15 |
| GSM2230846 |
distal kidney, feed with glucosinolates, fish 8 |
| GSM2230847 |
distal kidney, feed with glucosinolates, fish 9 |
| GSM2230848 |
liver, control feed, fish 28 |
| GSM2230849 |
liver, control feed, fish 29 |
| GSM2230850 |
liver, control feed, fish 3 |
| GSM2230851 |
liver, control feed, fish 31 |
| GSM2230852 |
liver, control feed, fish 36 |
| GSM2230853 |
liver, feed with glucosinolates, fish 12 |
| GSM2230854 |
liver, feed with glucosinolates, fish 14 |
| GSM2230855 |
liver, feed with glucosinolates, fish 15 |
| GSM2230856 |
liver, feed with glucosinolates, fish 8 |
| GSM2230857 |
liver, feed with glucosinolates, fish 9 |
| GSM2230858 |
muscle, control feed, fish 28 |
| GSM2230859 |
muscle, control feed, fish 29 |
| GSM2230860 |
muscle, control feed, fish 3 |
| GSM2230861 |
muscle, control feed, fish 31 |
| GSM2230862 |
muscle, control feed, fish 36 |
| GSM2230863 |
muscle, feed with glucosinolates, fish 12 |
| GSM2230864 |
muscle, feed with glucosinolates, fish 14 |
| GSM2230865 |
muscle, feed with glucosinolates, fish 15 |
| GSM2230866 |
muscle, feed with glucosinolates, fish 8 |
| GSM2230867 |
muscle, feed with glucosinolates, fish 9 |
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| Relations |
| BioProject |
PRJNA328589 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE84288_RAW.tar |
38.2 Mb |
(http)(custom) |
TAR (of GPR) |
| GSE84288_normalized.txt.gz |
1.4 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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