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| Status |
Public on Aug 22, 2016 |
| Title |
Memory-like CD8 T-cells sustain the immune response to chronic viral infections. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We have discovered a small subpopulation of virus-specific CD8 T-cells that sustains the T-cell response in chronic infections. These cells are defined by - and depend on - the expression of the transcription factor Tcf1 (T cell factor 1) and show key characteristics of central memory cells while lacking an effector signature. Unlike conventional memory cells, Tcf1+ T-cells display hallmarks of an “exhausted” phenotype, including the expression of certain inhibitory receptors.
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| Overall design |
Naive Tcf1-GFP+ P14 cells (Naive) were transferred into Vb5 recipient mice (CD45.1) prior to infection with LCMV clone 13 (c13). Tcf1-GFP+ P14 cells (chronic Tcf1+) and Tcf1-GFP- P14 cells (chronic Tcf1-) were flow sorted on day 28 post infection. Naive Tcf1-GFP+ P14 cells (Naive) were also transferred into C57BL/6 hosts (CD45.1.2) prior to infection with LCMV Armstrong (Arm). Tcf1-GFP+ P14 cells (memory Tcf1+) and Tcf1-GFP- P14 cells (memory Tcf1-) were flow sorted on day 28 post infection. Total RNA was extracted, cDNA libraries prepared and sequencing was performed using Illumina HiSeq 2500 technology.
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| Contributor(s) |
Charmoy M, Pais Ferreira D, Calderon-Copete S, Chennupati V, Pradervand S, Held W |
| Citation(s) |
27533016 |
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| Submission date |
Jul 01, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Sandra Calderon |
| Organization name |
University of Lausanne
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| Department |
Center for Integrative Genomics
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| Lab |
Lausanne Genomics Technologies Facility
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| Street address |
Génopode Building
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| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA327590 |
| SRA |
SRP077748 |
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