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| Status |
Public on Nov 15, 2017 |
| Title |
JUNB is a critical AP1 component for SMAD2/3 binding after TGFβ stimulation [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We performed SMAD2/3 ChIP-seq analysis in MCF10A MII cells. To validate whether the changes in SMAD2/3 binding to the genome indeed resulted in changes in target gene expression, we performed RNA-seq transcriptome analysis after short and long periods of TGFβ stimulation (0, 1.5h and 16h) in MII cells. In addition, we revealed that JUNB is a critical AP1 component for SMAD2/3 binding after TGFβ stimulation. To assess the significance of JUNB for TGFβ-SMAD-target genes on a genome-wide scale, we also performed RNA-seq transcriptome analysis in JUNB-knock-downed MII cells.
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| |
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| Overall design |
RNA-seq analysis in MCF10A MII cells with TGFβ stimulation and/or JUNB knock down
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| |
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| Contributor(s) |
Morikawa M, Sundqvist A, van Dam H, ten Dijke P |
| Citation(s) |
29186616 |
| |
| Submission date |
Jun 27, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Masato Morikawa |
| E-mail(s) |
morikawa-tky@umin.ac.jp
|
| Phone |
+81-3-3964-1211
|
| Organization name |
Teikyo University
|
| Department |
Advanced Comprehensive Research Organization
|
| Street address |
2-21-1 Kaga, Itabashi-ku
|
| City |
Tokyo |
| ZIP/Postal code |
173-0003 |
| Country |
Japan |
| |
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| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE83788 |
SMAD2/3 are redirected to novel sites in MCF10A MII after prolonged TGFβ stimulation |
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| Relations |
| BioProject |
PRJNA326979 |
| SRA |
SRP077355 |
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