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| Status |
Public on Mar 08, 2017 |
| Title |
Absolute quantification of B cell transcriptome using ERCC spiked-in RNA |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA-seq analysis of with known quantity of cell numbers and reference RNA (ERCC) to determine absolute abundance of endogenous mRNA abundances in B cells
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| |
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| Overall design |
WT B cells were prepared as described. 3 x 10^6 cells were harvested at indicated time points (naïve, 1.5h, 4.5h, 13.5hr and 25.5hr). We determined the amount of ERCC control needed to cover the whole dynamic range of endogenous mRNA abundance at each time point in a pilot experiment, and added the following amounts of ERCC control into 3 x 10^6 cells before RNA extraction: naïve, 1uL or 0.1uL; 1.5h and 4.5h, 0.5uL; 13.5hr, 1uL; and 25.5hr, 2uL.
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| |
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| Contributor(s) |
Jin HY, Xiao C |
| Citation(s) |
28241004 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AI087634 |
The Role of miR-17~92 in Autoimmune Diseases |
THE SCRIPPS RESEARCH INSTITUTE |
Changchun Xiao |
| R01 AI089854 |
Functional Analysis of MicroRNAs in Lymphocyte Development and Immune Tolerance |
THE SCRIPPS RESEARCH INSTITUTE |
Changchun Xiao |
| R01 AI121155 |
Regulation of T Follicular Helper Cell Differentiation by MicroRNAs |
THE SCRIPPS RESEARCH INSTITUTE |
Changchun Xiao |
| R56 AI110403 |
Regulation of T Follicular Helper Cell Differentiation by MicroRNAs |
THE SCRIPPS RESEARCH INSTITUTE |
Changchun Xiao |
| R56 AI121155 |
Regulation of T Follicular Helper Cell Differentiation by MicroRNAs |
THE SCRIPPS RESEARCH INSTITUTE |
Changchun Xiao |
|
| |
| Submission date |
Jun 27, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Changchun Xiao |
| E-mail(s) |
cxiao@scripps.edu
|
| Phone |
(858) 784-7640
|
| Organization name |
The Scripps Research Institute
|
| Department |
Immunology & Microbial Science
|
| Lab |
Changchun Xiao Lab
|
| Street address |
10550 North Torrey Pines Road
|
| City |
La Jolla |
| State/province |
California |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (14)
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| Relations |
| BioProject |
PRJNA326899 |
| SRA |
SRP077254 |
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