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| Status |
Public on Feb 20, 2021 |
| Title |
Binding site identification for the Epstein-Barr virus transcription factor Zta in epithelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Epstein-Barr virus (EBV) is present in a state of latency in infected memory B-cells and EBV-associated lymphoid and epithelial cancers. Cell stimulation or differentiation of infected B-cells and epithelial cells induces reactivation to the lytic replication cycle. In each cell type, the EBV transcription and replication factor Zta (BZLF1, EB1) plays a role in mediating the lytic cycle of EBV. Zta is a transcription factor that interacts directly with Zta response elements (ZREs) within viral and cellular genomes. Here we undertake chromatin-precipitation coupled to DNA-sequencing (ChIP-Seq) of Zta-associated DNA from cancer-derived epithelial cells. The analysis identified over 14,000 Zta-binding sites in the cellular genome. We assessed the impact of lytic cycle reactivation on changes in gene expression for a panel of Zta-associated cellular genes. Finally, we compared the Zta-binding sites identified in this study with those previously identified in B-cells and reveal substantial conservation in genes associated with Zta-binding sites.
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| Overall design |
Input and duplicate Zta ChIP Seq
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| Contributor(s) |
Sinclair AJ |
| Citation missing |
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| |
| Submission date |
Jun 14, 2016 |
| Last update date |
Sep 15, 2021 |
| Contact name |
Alison Jane Sinclair |
| E-mail(s) |
a.j.sinclair@sussex.ac.uk
|
| Organization name |
University of Sussex
|
| Department |
School of Life Sciences
|
| Lab |
Biochemistry and Bioscience
|
| Street address |
University of Sussex
|
| City |
Brighton |
| State/province |
E. Sussex |
| ZIP/Postal code |
BN1 9QG |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (3) |
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| Relations |
| Reanalysis of |
GSE58245 |
| BioProject |
PRJNA325662 |
| SRA |
SRP076554 |
