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| Status |
Public on Jun 10, 2017 |
| Title |
IL-33 and ST2 license beige and brown adipocytes for uncoupled respiration |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
For placental mammals, the transition from the in utero maternal environment to postnatal life requires the activation of thermogenesis to maintain their core temperature. This is primarily accomplished by induction of uncoupling protein 1 (UCP1) in brown and beige adipocytes, the principal sites for uncoupled respiration. Despite its importance, how placental mammals license their thermogenic adipocytes to participate in postnatal uncoupled respiration is not known. Here, we provide evidence that the 'alarmin' IL-33, a nuclear cytokine that activates type 2 immune responses, licenses brown and beige adipocytes for uncoupled respiration. We find that, in absence of IL-33 or ST2, beige and brown adipocytes develop normally but fail to express an appropriately spliced form of Ucp1 mRNA, resulting in absence of UCP1 protein, and impairment in uncoupled respiration and thermoregulation. Together, these data suggest that IL-33 and ST2 function as a developmental switch to license thermogenesis during the perinatal period.
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| |
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| Overall design |
mRNA profiles of brown adipose tissues and inguinal white adipose tissues from postnatal day 0.5 and 24, respectively, WT and IL-33 knockout mice.
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| |
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| Contributor(s) |
Chawla A, Odegaard JI, Lee MW, Sogawa Y |
| Citation(s) |
27453471 |
| |
| Submission date |
Jun 10, 2016 |
| Last update date |
Oct 15, 2019 |
| Contact name |
minwoo Lee |
| E-mail(s) |
wannasmile12@gmail.com
|
| Organization name |
University of California, San Francisco
|
| Department |
CVRI
|
| Lab |
Chawla lab
|
| Street address |
555 Mission bay blvd south
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (15)
|
|
| Relations |
| BioProject |
PRJNA325310 |
| SRA |
SRP076391 |
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