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| Status |
Public on Feb 27, 2017 |
| Title |
The FUS protein participates in circRNA biogenesis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
The RNA binding protein FUS exerts its function in several RNA biosynthetic processes; moreover, mutations leading to changes in its expression levels or alterations in cellular compartmentalization have been linked to the pathogenesis of Amyotrophic Lateral Sclerosis. Here, we describe an additional novel function of FUS, that is the control of back-splicing reactions which lead to circular RNA (circRNA) production. We identified circRNAs expressed in murine ES-derived motoneurons and then compared their levels in control versus FUS-/- cells. We discovered that a considerable number of circRNAs is affected by FUS levels.
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| Overall design |
Analysis of WT and FUS -/- mouse motorneurons. For each condition there is a triplicate of samples. In total 6 samples are analyzed.
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| Contributor(s) |
Modigliani SD, Errichelli L, Laneve P, Colantoni A, Legnini I, Capauto D, Rosa A, De Santis R, Scarfò R, Peruzzi G, Lei L, Caffarelli E, Shneider N, Morlando M, Bozzoni I |
| Citation(s) |
28358055 |
| |
| Submission date |
Jun 10, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Alessio Colantoni |
| E-mail(s) |
alessio.colantoni@uniroma1.it
|
| Organization name |
"Sapienza" University of Rome
|
| Department |
Dipartimento di Biologia e Biotecnologie "Charles Darwin"
|
| Lab |
Tartaglia Lab
|
| Street address |
Piazzale Aldo Moro, 5
|
| City |
Roma |
| State/province |
RM |
| ZIP/Postal code |
00185 |
| Country |
Italy |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA325303 |
| SRA |
SRP076387 |
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