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| Status |
Public on Jun 10, 2016 |
| Title |
Pharmacologic inhibition of host phosphodiesterase-4 improves isoniazid mediated clearance of Mycobacterium tuberculosis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
In this study, we evaluated the PK/PD and explored the efficacy of a PDE4 inhibitor, CC-11050 in a murine model of Mtb infection. Infected mouse lungs with or without CC-11050 treatment was also used to interrogate genome-wide transcriptional changes.
Standard aerosol infection of mice with MtbCDC1551, followed by enumeration of lung bacterial load, pathology and transcriptional changes were monitored upto 112 days post infection.
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| Overall design |
Mice were infected with Mtb and treatment with CC11050 was started at 14 days post-infection. Uninfected mice were included as control. At 28 days post-infection, mice from all 3 groups (uninfected, Mtb-infected untreated or CC11050 treated) were euthanized and lung tissue was collected to isolate total RNA and used in microarray experiments. (N=4 per group)
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| Contributor(s) |
Subbian S, Kaplan G |
| Citation(s) |
27379099 |
| |
| Submission date |
Jun 09, 2016 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Selvakumar Subbian |
| E-mail(s) |
subbiase@njms.rutgers.edu
|
| Phone |
9738543226
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| Organization name |
Rutgers Biomedical and Health Sciences, Rutgers The State University of NJ
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| Department |
New Jersey Medical School,
|
| Lab |
Mycobacterial Immunity and Pathogenesis
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| Street address |
225 Warren St, Rm# W250.Q
|
| City |
Newark |
| State/province |
NJ |
| ZIP/Postal code |
07103 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA325281 |
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