NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE83117 Query DataSets for GSE83117
Status Public on Jul 18, 2016
Title Inhibition of beta-catenin signalling in dermal fibroblasts enhances hair follicle regeneration during wound healing
Organism Mus musculus
Experiment type Expression profiling by array
Summary We performed gene expression profiling of P1 and P5 back and tail dermis to uncover potential explanations for the differences in HF formation at different ages and in different body sites.
 
Overall design To generate back and tail dermis microarray from P1 mouse (C57Bl6/CBA F1), tail skin was incubated in 5mM EDTA/PBS solution at 37°C, 1h, whereas back skin was incubated in dispase-trypsin solution at 37°C, 1h. Tail and back skin dermis were separated from the epidermis with forceps, washed once in PBS and digested as described above. Single-cell suspensions were washed 3x in PBS, before cells were lysed in Trizol (Invitrogen) and RNA was purified using Qiagen RNeasy columns. RNA was extracted from triplicate mice. cDNA was amplified from purified RNA and hybridized to Affymetrix MG430.2A arrays by the Cancer Research UK Patterson Institute Affymetrix Genechip Microarray Service. Array images were produced by the Affymetrix PICR 3000 scanner and analysed as Cell files using Genespring-13X (Agilent Technologies). RMA normalization was used and the bottom twentieth-percentile of genes (i.e., the 20% of genes with the lowest expression levels) were excluded from subsequent analysis.
 
Contributor(s) Gomez C, Pisco AO, Watt FM
Citation(s) 27287810
Submission date Jun 08, 2016
Last update date Feb 11, 2019
Contact name Angela Oliveira Pisco
E-mail(s) angela.pisco@czbiohub.org
Organization name CZ Biohub
Department Data Sciences
Street address 499 Illinois St
City San Francisco
State/province CA
ZIP/Postal code 94103
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (12)
GSM2193220 P1 Tail 4
GSM2193221 P1 Tail 5
GSM2193222 P1 Tail 6
Relations
BioProject PRJNA324815

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE83117_RAW.tar 44.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap