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| Status |
Public on Jun 08, 2016 |
| Title |
elli-1 gene expression array |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
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| Summary |
Germ cells contain non-membrane bound cytoplasmic organelles that help maintain germline integrity. In C. elegans they are called P granules; without them, the germline undergoes partial masculinization or aberrant differentiation. One key P-granule component is the Argonaute CSR-1, a small-RNA binding protein that antagonizes accumulation of sperm-specific transcripts in developing oocytes. Loss of CSR-1 or components of its pathway results in a very specific, enlarged P-granule phenotype. In a forward screen to identify mutants with abnormal P granules, ten alleles were recovered with csr-1 P-granule phenotypes, eight of which contain mutations in known CSR-1 pathway components (csr-1, ego-1, ekl-1, and drh-3). The remaining two alleles are in a novel gene now called elli-1 (enlarged germline granules). ELLI-1 is first expressed in primordial germ cells during mid-embryogenesis and continues to be expressed in the adult germline. While ELLI-1 forms cytoplasmic aggregates, they occasionally dock, but do not co-localize with P granules. Instead, the majority of ELLI-1 aggregates accumulate in the shared germline cytoplasm. In elli-1 mutants, several genes that promote RNAi and P-granule accumulation are upregulated, and embryonic lethality, sterility, and RNAi resistance in a hypomorphic drh-3 allele is enhanced. Like mutations in other CSR-1 pathway components, elli-1 is an enhancer of glp-1 sterility, suggesting that ELLI-1 functions with the CSR-1 pathway to modulate RNAi activity, P-granule accumulation, and post-transcriptional expression in the germline.
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| Overall design |
Four RNA samples from strain DUP9 elli-1(sam3) were compared to four RNA samples from strain DUP17 (control).
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| Contributor(s) |
Andralojc KM, Updike DL |
| Citation(s) |
28182654 |
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| Submission date |
Jun 07, 2016 |
| Last update date |
Jul 10, 2019 |
| Contact name |
Dustin Lynn Updike |
| E-mail(s) |
dupdike@mdibl.org
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| Phone |
2082889880
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| Organization name |
Mount Desert Island Biological Laboratory
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| Lab |
Updike Lab
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| Street address |
159 Old Bar Harbor Rd.
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| City |
Salisbury Cove |
| State/province |
ME |
| ZIP/Postal code |
04672 |
| Country |
USA |
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| Platforms (1) |
| GPL21109 |
Agilent-068300 ArrayXS_Celegans_OakLabs_06-2014_V2 (SystematicName version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA324647 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE82322_RAW.tar |
89.2 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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