GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE82282 Query DataSets for GSE82282
Status Public on Jun 07, 2016
Title MDM2 facilitates adipocyte differentiation through CRTC-mediated activation of STAT3
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary In this setup we have used RNA-seq to define which cAMP-induced genes are dependent on activity of the JAK kinases or MDM2. Also, we mapped binding of the coactivator CRTC2 to DNA upon forskolin stimulation.
Overall design Global gene expression analysis in 3T3-L1 preadipocytes as well as p53-/- and p53-/-;mdm2-/- MEFs treated with JAK inhibitor and/or forskolin. Furthermore, we have performed ChIP on CRTC2 in 3T3-L1 preadipocytes stimulated with forskolin and pretreated with either vehicle or the JAK inhibitor P6.
Contributor(s) Hallenborg P, Siersbæk M, Barrio-Hernandez I, Nielsen R, Kristiansen K, Mandrup S, Grøntved L, Blagoev B
Citation(s) 27362806
Submission date Jun 06, 2016
Last update date May 15, 2019
Contact name Susanne Mandrup
Phone +45 6550 2340
Organization name University of Southern Denmark
Department Biochemistry and Molecular Biology
Street address Campusvej 55
City Odense M
ZIP/Postal code 5230
Country Denmark
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (18)
GSM2187989 p53-/- MEFs, Vehicle, Rep 1
GSM2187990 p53-/- MEFs, Vehicle, Rep 2
GSM2187991 p53-/- MEFs, Forskolin, Rep 1
BioProject PRJNA324547
SRA SRP076165

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE82282_RAW.tar 489.2 Mb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap