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| Status |
Public on Jun 07, 2016 |
| Title |
MDM2 facilitates adipocyte differentiation through CRTC-mediated activation of STAT3 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
In this setup we have used RNA-seq to define which cAMP-induced genes are dependent on activity of the JAK kinases or MDM2. Also, we mapped binding of the coactivator CRTC2 to DNA upon forskolin stimulation.
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| Overall design |
Global gene expression analysis in 3T3-L1 preadipocytes as well as p53-/- and p53-/-;mdm2-/- MEFs treated with JAK inhibitor and/or forskolin. Furthermore, we have performed ChIP on CRTC2 in 3T3-L1 preadipocytes stimulated with forskolin and pretreated with either vehicle or the JAK inhibitor P6.
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| Contributor(s) |
Hallenborg P, Siersbæk M, Barrio-Hernandez I, Nielsen R, Kristiansen K, Mandrup S, Grøntved L, Blagoev B |
| Citation(s) |
27362806 |
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| Submission date |
Jun 06, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Susanne Mandrup |
| E-mail(s) |
s.mandrup@bmb.sdu.dk
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| Phone |
+45 6550 2340
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| Organization name |
University of Southern Denmark
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| Department |
Biochemistry and Molecular Biology
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| Street address |
Campusvej 55
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| City |
Odense M |
| ZIP/Postal code |
5230 |
| Country |
Denmark |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (18)
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| GSM2187992 |
p53-/- MEFs, Forskolin, Rep 2 |
| GSM2187993 |
p53-/-;mdm2-/- MEFs, Vehicle, Rep 1 |
| GSM2187994 |
p53-/-;mdm2-/- MEFs, Vehicle, Rep 2 |
| GSM2187995 |
p53-/-;mdm2-/- MEFs, Forskolin, Rep 1 |
| GSM2187996 |
p53-/-;mdm2-/- MEFs, Forskolin, Rep 2 |
| GSM2187997 |
3T3-L1, Vehicle, Rep1 |
| GSM2187998 |
3T3-L1, Vehicle, Rep2 |
| GSM2187999 |
3T3-L1, Forskolin, Rep1 |
| GSM2188000 |
3T3-L1, Forskolin, Rep2 |
| GSM2188001 |
3T3-L1, P6+Forskolin, Rep1 |
| GSM2188002 |
3T3-L1, P6+Forskolin, Rep2 |
| GSM2188003 |
3T3-L1, CRTC2-ChIP, Forskolin, Rep1 |
| GSM2188004 |
3T3-L1, CRTC2-ChIP, Forskolin, Rep2 |
| GSM2188005 |
3T3-L1, CRTC2-ChIP, P6+Forskolin, Rep1 |
| GSM2188006 |
3T3-L1, CRTC2-ChIP, P6+Forskolin, Rep2 |
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| Relations |
| BioProject |
PRJNA324547 |
| SRA |
SRP076165 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE82282_RAW.tar |
489.2 Mb |
(http)(custom) |
TAR (of BEDGRAPH) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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