GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE82260 Query DataSets for GSE82260
Status Public on Jul 28, 2016
Title ZMYND8 reads the dual histone mark H3K4me1-H3K14ac to antagonize the expression of metastasis-linked genes
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Histone acetylation, including acetylated H3K14 (H3K14ac), is generally linked to gene activation. Monomethylated histone H3 lysine 4 (H3K4me1), together with other gene-activating marks, denotes active genes. In contrast to usual gene-activating functions of H3K14ac and H3K4me1, we here show that the dual histone modification mark H3K4me1-H3K14ac is recognized by ZMYND8 (also called RACK7) and functions to counteract gene expression. We identified ZMYND8 as a transcriptional corepressor of the H3K4 demethylase JARID1D. ZMYND8 antagonizes the expression of metastasis-linked genes, and its knockdown increases the cellular invasiveness in vitro and in vivo. The plant homeodomain (PHD) and Bromodomain cassette in ZMYND8 mediates the combinatorial recognition of H3K4me1-H3K14ac and H3K4me0-H3K14ac by ZMYND8. These findings uncover an unexpected role for the signature H3K4me1-H3K14ac in attenuating gene expression and reveal a previously unknown metastasis-suppressive epigenetic mechanism in which ZMYND8's PHD-Bromo cassette couples H3K4me1-H3K14ac with repression of metastasis-linked genes.
Overall design i) ChIP-Seq data of ZMYND8, JARID1D, H3K4me1, H3K14ac, H3K4me3, and H3K27me3 in normal DU145 cells.
ii) ChIP-Seq data of H3K4me1 and H3K4me3 in shLuciferase-, shJARID1D-, or shZMYND8-treated DU145 cells.
iii) RNA-Seq data in shLuciferase-, shJARID1D-, or shZMYND8-treated DU145 cells.
Contributor(s) Lee MG, Li W
Citation(s) 27477906
Submission date Jun 03, 2016
Last update date May 15, 2019
Contact name JIE Lv
Organization name St Jude Children's Research Hospital
Department Department of Computational Biology
Street address 262 Danny Thomas Place
City Memphis
State/province TN
ZIP/Postal code 38105
Country USA
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (35)
GSM2187236 H3K14ac_ChIP-Seq
GSM2187237 H3K27me3_ChIP-Seq
GSM2187238 H3K4me1_ChIP-Seq_rep1
BioProject PRJNA324450
SRA SRP076126

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE82260_H3K14ac.bgsub.Fnor.wig.gz 107.9 Mb (ftp)(http) WIG
GSE82260_H3K27me3.bgsub.Fnor.wig.gz 80.5 Mb (ftp)(http) WIG
GSE82260_H3K4me1-shControl.bgsub.Fnor.wig.gz 112.2 Mb (ftp)(http) WIG
GSE82260_H3K4me1-shJARID1D.bgsub.Fnor.wig.gz 110.2 Mb (ftp)(http) WIG
GSE82260_H3K4me1-shZMYND8.bgsub.Fnor.wig.gz 116.4 Mb (ftp)(http) WIG
GSE82260_H3K4me1.bgsub.Fnor.wig.gz 147.7 Mb (ftp)(http) WIG
GSE82260_H3K4me3-shControl.bgsub.Fnor.wig.gz 64.6 Mb (ftp)(http) WIG
GSE82260_H3K4me3-shJARID1D.bgsub.Fnor.wig.gz 62.3 Mb (ftp)(http) WIG
GSE82260_H3K4me3-shZMYND8.bgsub.Fnor.wig.gz 59.9 Mb (ftp)(http) WIG
GSE82260_H3K4me3.bgsub.Fnor.wig.gz 26.3 Mb (ftp)(http) WIG
GSE82260_Input-Control.uniq.wig.gz 48.3 Mb (ftp)(http) WIG
GSE82260_Input-shJARID1D.uniq.wig.gz 43.9 Mb (ftp)(http) WIG
GSE82260_Input-shZMYND8.uniq.wig.gz 39.1 Mb (ftp)(http) WIG
GSE82260_JARID1D.bgsub.Fnor.wig.gz 188.6 Mb (ftp)(http) WIG
GSE82260_JARID1D_input.Fnor.wig.gz 124.3 Mb (ftp)(http) WIG
GSE82260_ZMYND8.bgsub.Fnor.wig.gz 149.8 Mb (ftp)(http) WIG
GSE82260_ZMYND8_input.Fnor.wig.gz 143.4 Mb (ftp)(http) WIG
GSE82260_genes.count_tracking.txt.gz 1.4 Mb (ftp)(http) TXT
GSE82260_input.Fnor.wig.gz 78.1 Mb (ftp)(http) WIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap