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| Status |
Public on Jul 21, 2016 |
| Title |
Determination of the RNA linked to the paraspeckle in the GH4C1 cell line |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The goal of this study was to determine the identity of the RNA linked to the paraspeckles in the rat GH4C1 cell line.
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| Overall design |
GH4C1 cell were fixed with 1% paraformaldehyde in PBS. Then nuclei were purified, lysed and sonicated. RNA pull-down was performed using two antisense DNA biotinylated oligonucleotide probes that target the lncRNA Neat1. Streptavidin-magnetic beads were added to hybridization reaction and complexes were captured by magnets. RNA was isolated using NucleoSpin®RNA XS (Macherey-Nagel).
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| Contributor(s) |
Torres M, Becquet D, Blanchard M, Guillen S, Boyer B, Moreno M, Franc J, François-Bellan A |
| Citation(s) |
27441387 |
| |
| Submission date |
May 27, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Anne-Marie François-Bellan |
| E-mail(s) |
anne-marie.francois@univ-amu.fr
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| Organization name |
CNRS-AMU
|
| Department |
Facute de Medecine
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| Lab |
INP UMR 7051 CNRS-AMU
|
| Street address |
27 Bd Jean Moulin
|
| City |
Marseille |
| ZIP/Postal code |
13005 |
| Country |
France |
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| Platforms (1) |
| GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA323540 |
| SRA |
SRP075804 |
