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| Status |
Public on Jan 01, 2017 |
| Title |
RNASequencing of nf-yc, hy5, and nf-yc hy5 mutants during photomorphogenic growth in Arabidopsis thaliana. |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: The purpose of this RNA Sequencing project was to investigate the transcriptional regulatory relationship between NF-YC3/4/9 and HY5
Methods: Total RNA was isolated, and then poly-A purified. 100ng of starting RNA was used to generate RNASeq libraries using the NEXTflex Illumina qRNA-Seq Library Prep Kit, and sequenced on an Illumina HiSeq2500 machine.
Results: NF-YC3/4/9 and HY5 have both shared and independent regulatory targets.
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| Overall design |
mRNA profiles of 7-day old Wild Type (WT), nf-yc triple (Q3), hy5, and nf-yc triple hy5 (Q3hy5) seedlings grown in continuous white light were generated from two biological replicates through paired end deep sequencing on an Illumina HiSeq2500 platform.
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| |
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| Contributor(s) |
Myers ZA, Kumimoto RW, Risinger JR, Holt BF |
| Citation(s) |
27685091 |
| |
| Submission date |
May 24, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Ben F Holt |
| E-mail(s) |
benholt@ou.edu
|
| Phone |
4053259092
|
| Organization name |
University of Oklahoma
|
| Department |
Microbiology and Plant Biology
|
| Lab |
Holt Lab
|
| Street address |
770 Van Vleet Oval
|
| City |
Norman |
| State/province |
OK |
| ZIP/Postal code |
73019 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17639 |
Illumina HiSeq 2500 (Arabidopsis thaliana) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA322671 |
| SRA |
SRP075658 |
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