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Series GSE81818 Query DataSets for GSE81818
Status Public on Feb 14, 2018
Title Profiling γ-irradiated invasive Plasmodium falciparum merozoites using a systems biology approach
Platform organisms Plasmodium falciparum; Anopheles gambiae
Sample organism Plasmodium falciparum
Experiment type Expression profiling by array
Summary Plasmodium falciparum malaria severely impacts human health. In order to broaden our understanding of merozoite invasion of erythrocytes which is responsible for clinical disease, a P. falciparum γ-irradiated "long-lived merozoite" (LLM) line was investigated. Cell-sieve purified LLM invaded human erythrocytes with an improved efficiency of 10- to 300-fold greater than wild-type (WT) parasites. A comparison of their genomes identified limited changes in the open reading frame of LLM; while only marginal differences were observed in the transcriptomes. Analysis of their proteomes by quantitative mass-spectrometry identified 446 out of 981 proteins of known or unknown function with a significant change in protein abundance (ANOVA p < 0.05). Furthermore, the relative molar concentration of nearly 1100 merozoite proteins was established. Unfortunately, a specific change being responsible for the LLM phenotype was not identified. However, immunoblot analyses of LLM lysates showed proteolytic processing of some proteins of the MSP1 complex and AMA1 were delayed, suggesting that this delayed proteolysis positively impacted merozoite viability and subsequent invasion.
 
Overall design Wild type vs. long-lived merozoite (LLM) Plasmodium falciparum.
 
Contributor(s) Kumar K, Srinivasan P, Nold MJ, Moch K, Reiter K, Sturdevant DE, Squires RB, Herrera R, Nagarajan V, Rayner JC, Porcella SF, Geromanos SJ, Haynes JD, Narum DL
Citation(s) 29215067
Submission date May 24, 2016
Last update date May 20, 2018
Contact name Dan Sturdevant
E-mail(s) dsturdevant@niaid.nih.gov
Phone 4063639248
Organization name NIH
Department NIAID
Lab RTS
Street address 903 S 4th street
City Hamilton
State/province MT
ZIP/Postal code 59840
Country USA
 
Platforms (1)
GPL1321 [Plasmodium_Anopheles] Affymetrix Plasmodium/Anopheles Genome Array
Samples (32)
GSM2176287 Wild type Plasmodium falciparum rep 1
GSM2176288 Wild type Plasmodium falciparum rep 2
GSM2176289 Wild type Plasmodium falciparum rep 3
Relations
BioProject PRJNA322624

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE81818_RAW.tar 56.8 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file
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