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| Status |
Public on Mar 27, 2017 |
| Title |
A comprehensive analysis of gene expression patterns in wild-type and Batf2-/- bone marrow-derived macrophages (BMMφ) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We previously identified TLR-independent expression of 4933430F08Rik, encoding Batf2, in T. cruzi-infected bone marrow-derived dendritic cells (BMDCs) (Kayama et al., 2009). To determine the functions of Batf2 in innate immune responses, we performed a comprehensive gene expression analysis in wild-type and Batf2-/- bone marrow-derived macrophages (BMMφ). RNA-seq analysis showed that 98 genes are upregulated in Batf2-/- BMMφ stimulated with LPS following IFN-γ treatment, when compared with that in wild-type cells. Among these genes, we focused on Il23a, encoding IL-23p19, because IL-23 is able to promote expression of Il17a in Th17 cells.
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| Overall design |
mRNA of wild-type and Batf2-/- BMMφ were subjected to deep sequencing profiling using Illumina HiSeq 2000.
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| Contributor(s) |
Kitada S, Kayama H, Okuzaki D, Koga R, Kobayashi M, Arima Y, Murakami M, Takeda K |
| Citation(s) |
28356392 |
| |
| Submission date |
May 22, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Daisuke Okuzaki |
| E-mail(s) |
dokuzaki@biken.osaka-u.ac.jp
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| Phone |
+81-6-6879-4935
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| Organization name |
Osaka univ.
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| Department |
Immunology Frontier Research Center
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| Lab |
Human Immunology （Single Cell Genomics)
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| Street address |
Yamadaoka 3-1
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| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA322420 |
| SRA |
SRP075525 |
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