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| Status |
Public on May 18, 2017 |
| Title |
Expression data of nuclear mRNA export mutant rae1-167 cells |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Schizosaccharomyces pombe |
| Experiment type |
Expression profiling by array
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| Summary |
rae1 is an essential gene and encodes one of nuclear pore complex. rae1-167 mutant cells show rapid accumulation of polyA-RNA in the nucleus at 36°C followed by protein accumulation, suggesting that accumulated nuclear mRNA influences nucelar cytooplasmic transport.
We used microarrays to compare the overall gene expression of rae1-167 cells grown at 36°C with that at 25°C.
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| Overall design |
rae1-167 cells grown in YE4S medium were harvested for RNA extraction and hybridization on Affymetrix microarray. In order to characterize transcriptional profiling of rae1-167 cells at 36°C, we carried out the genome-wide gene expression analysis of rae1-167 cells.
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| Contributor(s) |
Kume K, Kanai M |
| Citation(s) |
28545058 |
| Submission date |
May 20, 2016 |
| Last update date |
Aug 17, 2017 |
| Contact name |
MUNEYOSHI KANAI |
| E-mail(s) |
kanai@nrib.go.jp
|
| Organization name |
National Research Institute of Brewing
|
| Street address |
3-7-1
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| City |
Kagamiyama, Higashi-Hiroshima |
| ZIP/Postal code |
739-0046 |
| Country |
Japan |
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| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA322255 |
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