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Series GSE81600 Query DataSets for GSE81600
Status Public on Aug 03, 2016
Title A long noncoding RNA regulates sister chromatid cohesion [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Long noncoding RNAs (lncRNAs) have appeared to be involved in the most diverse cellular processes through multiple mechanisms. Here we describe a previously uncharacterized human lncRNA, CONCR (cohesion regulator noncoding RNA), transcriptionally activated by MYC, which is upregulated in multiple cancer types. The expression of CONCR is cell cycle-regulated, and it is required for cell cycle progression and DNA replication. Moreover, cells depleted of CONCR show severe defects in sister chromatid cohesion, suggesting an essential role for CONCR in cohesion establishment during cell division. CONCR interacts with and regulates the activity of DDX11, a DNA-dependent ATPase and helicase involved in DNA replication. These findings suggest a novel mechanism of action for CONCR in the modulation of DDX11 enzymatic activity, unveiling the direct involvement of a lncRNA in the establishment of sister chromatid cohesion.
Overall design Characterization of the function of the long noncoding RNA CONCR. Analysis of DDX11 chromatin binding by ChIP-seq in the presence or absence of CONCR.
Contributor(s) Marchese FP, Grossi E, Marín-Béjar O, Bharti SK, Raimondi I, González J, Martínez-Herrera DJ, Athie A, Amadoz A, Brosh RM, Huarte M
Citation(s) 27477908
Submission date May 18, 2016
Last update date May 15, 2019
Contact name Maite Huarte
Organization name CIMA
Street address c/ Avd Pio XII, 55
City Pamplona
State/province Navarra
ZIP/Postal code 31008
Country Spain
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (6)
GSM2157830 DDX11_ChIP-Seq_in_CONCR_KD_rep1
GSM2157831 DDX11_ChIP-Seq_in_CONCR_KD_rep2
GSM2157832 CONCR_KD_input_DNA
This SubSeries is part of SuperSeries:
GSE81602 A long noncoding RNA regulates sister chromatid cohesion
BioProject PRJNA322066
SRA SRP075372

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Supplementary file Size Download File type/resource
GSE81600_RAW.tar 1.3 Gb (http)(custom) TAR (of BEDGRAPH)
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Raw data are available in SRA
Processed data provided as supplementary file

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