 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Sep 10, 2017 |
| Title |
Expression data generated by full-length and truncated syndecan-1 overexpression in B6FS fibrosarcoma cell line |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To dissect the functions of syndecan-1 in the nucleus, and separate them from functions related to the cell-surface, we transfected fibrosarcoma cells with two constructs: one encoding the full-length syndecan-1, which translocates to the nucleus and another encoding syndecan-1 lacking the RMKKK nuclear localization signal with hampered nuclear translocation.
Using this model system, we combined global transcriptomic and proteomic approaches in order to disclose the molecular mechanisms governing the nuclear translocation and its related functions. Our analysis identified several genes and pathways related to the nuclear compartment TGF-β pathway was activated by nuclear syndecan-1 and three genes were significantly altered by NLS deletion: NEK11 , DOCK8 , and EGR-1 as potential candidates coupled to syndecan-1 in the nucleus.
|
| |
|
| Overall design |
Gene expression profiles (Human Gene 1.1 ST) of fibrosarcoma cells were studied in cells overexpressing full length syndecan-1 (N=3), syndecan-1 lacking its NLS (N=3) and empty vector control cells (N=3)
|
| |
|
| Contributor(s) |
Szatmári T, Mundt F, Brodin D, Dobra K |
| Citation(s) |
29216821 |
| |
| Submission date |
May 17, 2016 |
| Last update date |
Dec 10, 2017 |
| Contact name |
Tünde Szatmári |
| E-mail(s) |
tszatmari@yahoo.com
|
| Organization name |
Karolinska Institute
|
| Department |
Laboratory Medicine
|
| Street address |
F42 Karolinska Universitetssjukhuset
|
| City |
Huddinge |
| State/province |
Stockholm |
| ZIP/Postal code |
14186 |
| Country |
Sweden |
| |
|
| Platforms (1) |
| GPL11532 |
[HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version] |
|
| Samples (9)
|
| GSM2154897 |
CELLS OVEREXPRESSING FULL LENGTH SYNDECAN-1, biological replicate 1 |
| GSM2154898 |
CELLS OVEREXPRESSING FULL LENGTH SYNDECAN-1, biological replicate 2 |
| GSM2154899 |
CELLS OVEREXPRESSING FULL LENGTH SYNDECAN-1, biological replicate 3 |
| GSM2154900 |
CELLS OVEREXPRESSING SYNDECAN-1 LACKING RMKKKdel NUCLEAR LOCALIZATION SIGNAL, biological replicate1 |
| GSM2154901 |
CELLS OVEREXPRESSING SYNDECAN-1 LACKING RMKKKdel NUCLEAR LOCALIZATION SIGNAL, biological replicate2 |
| GSM2154902 |
CELLS OVEREXPRESSING SYNDECAN-1 LACKING RMKKKdel NUCLEAR LOCALIZATION SIGNAL, biological replicate3 |
| GSM2154903 |
VECTOR CONTROL CELLS, biological replicate1 |
| GSM2154904 |
VECTOR CONTROL CELLS, biological replicate2 |
| GSM2154905 |
VECTOR CONTROL CELLS, biological replicate3 |
|
| Relations |
| BioProject |
PRJNA321830 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE81504_RAW.tar |
40.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...