|
| Status |
Public on Sep 20, 2016 |
| Title |
Sirtuin 1 Promotes Naïve Pluripotency by Deacetylation of Oct4 (RNA-seq) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The enhancer landscape is dramatically restructured as naïve preimplantation epiblasts transition to the post-implantation state of primed pluripotency. A key factor in this process is Otx2, which is upregulated during the early stages of this transition and ultimately recruits Oct4 to a different set of enhancers. In this study we discover that the acetylation status of Oct4 regulates the induction of the primed pluripotency gene network. Maintenance of the naïve state requires Oct4 deacetylation by the NAD-dependent deacetylase, SirT1. The activity of SirT1 is reduced during the naïve to primed transition, thereby increasing the acetylation of Oct4 and promoting its binding to an Otx2 enhancer to induce Otx2 expression. Induction of Otx2 causes the reorganization of acetylated Oct4 and results in the induction of the primed pluripotency gene network. Regulation of Oct4 by SirT1 may link stem cell development to environmental conditions and provide strategies to manipulate epiblast cell state.
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| Overall design |
Transcription profile to compare the expression changes during the naïve (ESCs) to primed (EpiLCs) transition to the expression changes caused by Sirt1 loss and Otx2 knockdown.
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| |
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| Contributor(s) |
Williams E, Guarente L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 16, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Eric Williams |
| Organization name |
Massachusetts Institute of Technology
|
| Department |
Biology
|
| Lab |
Leonard Guarente
|
| Street address |
400 Main St; 68-288
|
| City |
Cambridge |
| State/province |
Ma |
| ZIP/Postal code |
02139 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA321759 |
| SRA |
SRP075247 |
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