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| Status |
Public on Aug 22, 2016 |
| Title |
Regional expression of X. tropicalis transcription factors in early gastrula embryos |
| Organism |
Xenopus tropicalis |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: We wished to obtain spatial expression profiles of transcription factor gene expression and expression of other genes.
Methods: Embryos were dissected and RNA-seq was performed to determine expression levels of genes. Comparisons between explanted regions permits determination of spatial enrichment of gene expression.
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| |
|
| Overall design |
mRNA-seq performed on 5 dissected regions of NF stage 10.5 X. tropicalis embryos and stage-matched sibling whole embryos. Experiment performed in duplicate.
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| |
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| Contributor(s) |
Blitz IL, Paraiso KD |
| Citation(s) |
27475627 |
| |
| Submission date |
May 16, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Kitt D. Paraiso |
| E-mail(s) |
kparaiso@uci.edu
|
| Phone |
949-824-7950
|
| Organization name |
University of California, Irvine
|
| Department |
Department of Developmental and Cell Biology
|
| Lab |
Ken W.Y. Cho
|
| Street address |
4410 Nat Sci II, University of California, Irvine
|
| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92697 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL21874 |
Illumina HiSeq 2000 (Xenopus tropicalis) |
| GPL21875 |
Illumina HiSeq 2500 (Xenopus tropicalis) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA321667 |
| SRA |
SRP075201 |
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