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Series GSE81434 Query DataSets for GSE81434
Status Public on Jun 30, 2016
Title Piwi modulates chromatin accessibility by regulating multiple factors including histone H1 to repress transposons
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary PIWI-interacting RNAs (piRNAs) mediate transposable element (TE) silencing at the transcriptional or post-transcriptional level in animal gonads. In the Drosophila ovary, Piwi–piRNA complexes (Piwi–piRISCs) repress TE transcription by modifying the chromatin state, such as H3K9me3 marks. Here, we demonstrate that Piwi physically interacts with linker histone H1. Depletion of Piwi decreases H1 density on target loci, leading to TE derepression. Loss of H1 results in gain of chromatin accessibility at target loci without affecting H3K9me3 and heterochromatin protein 1a (HP1a) density at the same loci. Piwi-mediated TE silencing also requires HP1a by regulating chromatin accessibility through its association with target loci. Thus, Piwi–piRISCs require both H1 and HP1a to repress TEs, and the silencing is correlated with the state of chromatin formation rather than H3K9me3 marks. These findings suggest that Piwi–piRISCs regulate the interaction of chromatin components with target loci to maintain silencing of the TE state through the modulation of chromatin accessibility.
Overall design RNA levels, H1 and H3K9me3 occupancy, chromatin accessibility, and Piwi-associated small RNA levels in ovarian somatic cells (OSC) depleted of piRNA pathway components and H1.
Contributor(s) Iwasaki YW, Saito K, Siomi H
Citation(s) 27425411
Submission date May 13, 2016
Last update date Jul 03, 2021
Contact name Yuka W. Iwasaki
Organization name Keio University School of Medicine
Department Department of Molecular Biology
Lab Siomi Lab
Street address 35 Shinanomachi, Shinjuku-ku,
City Tokyo
ZIP/Postal code 160-8582
Country Japan
Platforms (2)
GPL16479 Illumina MiSeq (Drosophila melanogaster)
GPL17275 Illumina HiSeq 2500 (Drosophila melanogaster)
Samples (34)
GSM2152419 RNA-seq from Control-KD
GSM2152420 RNA-seq from Piwi-KD
GSM2152421 RNA-seq from H1-KD
BioProject PRJNA321533
SRA SRP075098

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE81434_processed_ATACseq.txt.gz 4.7 Kb (ftp)(http) TXT
GSE81434_processed_ChIPseqIN_1.txt.gz 3.0 Kb (ftp)(http) TXT
GSE81434_processed_ChIPseqIN_2.txt.gz 2.2 Kb (ftp)(http) TXT
GSE81434_processed_ChIPseqIN_3.txt.gz 2.2 Kb (ftp)(http) TXT
GSE81434_processed_H1ChIPseq_1.txt.gz 2.2 Kb (ftp)(http) TXT
GSE81434_processed_H1ChIPseq_2.txt.gz 2.2 Kb (ftp)(http) TXT
GSE81434_processed_H1ChIPseq_3.txt.gz 2.2 Kb (ftp)(http) TXT
GSE81434_processed_H3K9ChIPseq.txt.gz 3.0 Kb (ftp)(http) TXT
GSE81434_processed_HP1ChIPseq.txt.gz 3.0 Kb (ftp)(http) TXT
GSE81434_processed_RNAseq.txt.gz 2.9 Kb (ftp)(http) TXT
GSE81434_processed_smRNAseq.txt.gz 2.2 Kb (ftp)(http) TXT
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Processed data are available on Series record

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