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| Status |
Public on May 01, 2019 |
| Title |
Immunomodulatory mechanism of Cordyceps militaris polypeptide through regulating gene in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Cordyceps militaris and Cordyceps sinensis belong to belong to the same genus, but the different species, with immunity improvement, antibacterial, and antihypertensive effect, and the studies on the functions of Cordyceps militaris mainly focus on those of its polysaccharides and polypeptides. The latest studies have found that some of the polypeptides with immunomodulatory effect can widely regulate immune functions at multiple levels, improve immunity, and enhance immune functions to ensure the healthy body, showing an important significance. Cordyceps militaris polypeptide prepared with the enzymolysis method was taken as the research object in this study. The differentially expressed genes and the related cell signal transduction pathway were screened by mRNA expression microarray. STEM 1.3.6 software was used for the clustering of the gene functions, and David and KEGG database were applied for the analysis of the related functions. 1748 differentially expressed genes were selected finally and three of them were validated by qPCR. The results showed that gene Hist1h2bp, Ctsg, and Elane were involved in the regulation of Cordyceps militaris on the immune activity of mice. Gene Hist1h2bp, Ctsg and Elan may be the potential targets of Cordyceps militaris polypeptide, which may provide an important theory basis for the further research and development of Cordyceps militaris polypeptide.
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| Overall design |
The experimental animals were fed under the conditions with a relative humidity of 40-70%, at 20±1℃ and in a sterile environment, with ad libitum access to water and food. After they adapted to the environment for one week, the mice were randomly divided into blank control group, model group, and low-, medium- and high-dose Cordyceps militaris polypeptide (CMP) groups. Mice in low-, medium- and high-dose Cordyceps militaris polypeptide were orally given 32, 160 and 800 mg/kg once daily continuously for 45 days, respectively, and those in the blank control group and model group were given equal volume of distilled water in the same way. In addition to those in the blank control group, mice in the other groups were injected with 40 mg/kg of cyclophosphamide intraperitoneally once daily, successively for 2 day for the establishment of mice immunodeficiency model. The mice's body weights were measured every week regularly for the observation on changes in their body weights and their general states were also observed at the same time. We choose best group(high-dose group) to do mRNA expression microarray.
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| Contributor(s) |
xu g, yuan g |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
May 12, 2016 |
| Last update date |
May 02, 2019 |
| Contact name |
guangyu xu |
| E-mail(s) |
xuguangyu2005@163.com
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| Organization name |
College of Pharmacy, Beihua University
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| Street address |
Binjiang Road 3999,jilin city,132013
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| City |
jilin city |
| ZIP/Postal code |
+860432 |
| Country |
China |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (6)
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| GSM2151620 |
spleen organization 2d 40 mg/kg replicate 1 |
| GSM2151621 |
spleen organization 2d 40 mg/kg replicate 2 |
| GSM2151622 |
spleen organization 2d 40 mg/kg replicate 3 |
| GSM2151623 |
spleen organization 45d 800 mg/kg/d replicate 1 |
| GSM2151624 |
spleen organization 45d 800 mg/kg/d replicate 2 |
| GSM2151625 |
spleen organization 45d 800 mg/kg/d replicate 3 |
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| Relations |
| BioProject |
PRJNA321726 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE81389_RAW.tar |
13.0 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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