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| Status |
Public on Jul 04, 2016 |
| Title |
Profiling of mouse muscle stem cells aging and activation upon skeletal muscle injury |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Utilizing glycerol intramuscular injections in M. musculus provide a models of skeletal muscle damage followed by skeletal muscle regeneration. In particular, glycerol-induced muscle injury triggers accute activation of skeletal muscle stem cells, called satellite cells. However, aging dramatically impairs the regenerative capacity of satellite cells. We characterized genome-wide expression profiles of young and old satellite cells in the non-proliferative and activated state, freshly isolated to non-injured or damaged muscles, respectively. Our goal was to uncover new regulatory signaling specific to satellite cells entry into the activation and myogenic program that are affected with age.
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| Overall design |
Satellite cells were isolated in either quiescent / non-proliferative or activated state from uninjured or 3 days after glycerol-induced injury of tibialis anterior, gastrocnemius and quadriceps, respectively. Young (2-4 months old) and old (20-24 months old) wildtype C57BL/6J male were used, with five to six biological replicates per group.
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| Contributor(s) |
Lukjanenko L, Migliavacca E, Karaz S, Metairon S, Raymond F, Bentzinger F, Feige JN |
| Citation(s) |
27376579, 35245177 |
| |
| Submission date |
May 04, 2016 |
| Last update date |
Mar 30, 2022 |
| Contact name |
Jerome Feige |
| Organization name |
Nestle Institute Of Health Sciences
|
| Department |
Musculo-Skeletal Health
|
| Street address |
EPFL Innovation Park, Building H
|
| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (23)
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| Relations |
| BioProject |
PRJNA320484 |
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