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Series GSE81095 Query DataSets for GSE81095
Status Public on Jan 01, 2017
Title RNA Sequencing Data in differentiating mouse embryonic stem cells [day2 and 2.5 in differentiation]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary ES cell lines were established from mouse embryos, which were homozygous for the Trim33-flox allele and carried the UbcCreERT2 transgene. Cells were cultured without feeder cells in the presence of LIF and 2i. Embryoid bodies (EBs) were generated using the ATCC protocol on low attachment dishes under differentiating conditions. EBs were induced with Tamoxifen at day 1 and harvested at days 2 and 2.5, respectively.
 
Overall design Investigate differentially expressed genes in control and Trim33-deficient embryoid bodies derived from mouse embryonic stem cells
 
Contributor(s) Kaartinen VM, Rajderkar S
Citation(s) 29090280
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 DE013085 The Role of TGF-Beta3 in Palatogenesis REGENTS OF THE UNIVERSITY OF MICHIGAN - ANN ARBOR VESA M KAARTINEN
R01 HL074862 Activin Receptor-Like Kinase-2 in Cardiac Morphogenesis REGENTS OF THE UNIVERSITY OF MICHIGAN - ANN ARBOR VESA M KAARTINEN
Submission date May 04, 2016
Last update date May 15, 2019
Contact name Vesa Matti Kaartinen
E-mail(s) vesak@umich.edu
Phone 734 615 4726
Organization name University of Michigan
Department BMS
Lab Kaartinen
Street address 1011 N. University
City Ann Arbor
State/province MI
ZIP/Postal code 48109
Country USA
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (12)
GSM2142836 Day 2 Control rep1
GSM2142837 Day 2 Control rep2
GSM2142838 Day 2 Control rep3
Relations
BioProject PRJNA320482
SRA SRP074352

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE81095_RAW.tar 1.2 Mb (http)(custom) TAR (of TXT)
GSE81095_Results_Day2EB_SR.xlsx 108.6 Kb (ftp)(http) XLSX
GSE81095_Results_Day2_5EB_SR.xls.gz 439.1 Kb (ftp)(http) XLS
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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