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Series GSE80708 Query DataSets for GSE80708
Status Public on Apr 27, 2017
Title An ES cell–specific NuRD complex functions through interaction with Wdr5
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The Nucleosome Remodeling and Deacetylase (NuRD) complex is a chromatin regulatory complex that functions as a transcriptional co-repressor in metazoans. The NuRD subunit Mbd3 is essential for targeting and assembly of a functional NuRD complex as well as embryonic stem cell (ESC) pluripotency. Three Mbd3 isoforms (Mbd3a, Mbd3b, and Mbd3c) are expressed in mouse. Here, we find that the Mbd3c isoform contains a unique 50–amino acid N-terminal region that is necessary for Mbd3c to specifically interact with the histone H3 binding protein Wdr5. Domain analyses of Wdr5 reveal that the Wdr5 H3K4/MLL binding pocket is required for interaction with Mbd3c. We find that while Mbd3c KO ESCs differentiate normally, Mbd3c is redundant with the Mbd3a and Mbd3b isoforms in regulation of gene expression and ESC pluripotency, with the unique Mbd3c N-terminus required for this redundancy. Together, our data characterize a novel NuRD complex variant that functions specifically in ESCs
Overall design Gene expression profiles generated by RNA-seq of Mbd3 isoform mutant mouse embryonic stem cells
Contributor(s) Ee L, McCannell KN, Tang Y, Fernandes N, Hardy WR, Green MR, Chu F, Fazzio TG
Citation(s) 28528697
Submission date Apr 27, 2016
Last update date May 15, 2019
Contact name Thomas Fazzio
Organization name University of Massachusetts Medical School
Street address 364 Plantation Street
City Worcester
State/province Massachusetts
ZIP/Postal code 01605
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (10)
GSM2133798 E14 WT rep1
GSM2133799 E14 WT rep2
GSM2133800 Mbd3ab KO rep1
BioProject PRJNA319759
SRA SRP074044

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE80708_ABCKO_sig.txt.gz 755.8 Kb (ftp)(http) TXT
GSE80708_ABKO_sig.txt.gz 43.2 Kb (ftp)(http) TXT
GSE80708_ABctr_sig.txt.gz 562.1 Kb (ftp)(http) TXT
GSE80708_CKO_sig.txt.gz 3.9 Kb (ftp)(http) TXT
GSE80708_RAW.tar 583.1 Mb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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