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Series GSE80597 Query DataSets for GSE80597
Status Public on Jan 06, 2017
Title Fetal and adult progenitors give rise to unique populations of CD8+ T cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary Neonatal CD8+ T cells are unable to form long-lived memory cells in response to infection due to cell-intrinsic reasons; however, the underlying basis for this difference is unclear. Here, using intrathymic transfers with fetal and adult progenitor cells, in conjunction with gene expression profiling and functional assays, we demonstrate that because neonatal CD8+ T cells are derived from a distinct progenitor cell population, they behave differently than adult cells from the time they are generated. These progenitor cells have higher Lin28 expression than adult progenitor cells. We found that adult CD8+ T cells generated from progenitor cells with induced Lin28 expression do not form memory cells, similar to neonatal cells. These findings suggest that neonatal and adult CD8+ T cells belong to separate lineages of CD8+ T cells and potentially explains why it is challenging to elicit memory CD8+ T cells in early life.
Overall design Gene expression analysis of adult and neonatal CD8+ T cells in the thymus, the spleen, and splenic cell subsets (naïve and innate memory). Small RNA analysis of CD8+ T cells expressing exogenous Lin28b.
Contributor(s) Wissink EM, Grimson A
Citation(s) 28034872
Submission date Apr 23, 2016
Last update date May 15, 2019
Contact name Andrew Grimson
Organization name Cornell University
Department Molecular Biology & Genetics
Street address 445 Biotechnology Building
City Ithaca
State/province NY
ZIP/Postal code 14853
Country USA
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (10)
GSM2131218 Adult_thymus
GSM2131219 Adult_spleen
GSM2131220 Adult_naive
BioProject PRJNA319424
SRA SRP073753

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Supplementary file Size Download File type/resource
GSE80597_Processed_data_cuffdiff_output.txt.gz 20.9 Mb (ftp)(http) TXT
GSE80597_miRNAs_expressed_iLin28b_WT.txt.gz 21.4 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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