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| Status |
Public on Nov 28, 2007 |
| Title |
Gene expression in a resistance artery in 2 models of hypertension in the rat |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
We investigated morphometric structure and gene expression by microarray analysis in a small diameter artery, branch of the saphenous artery (a resistance artery), in representative models of renin-angiotensin system (RAS)-dependent and glucocorticoid hypertension, using the spontaneously hypertensive rat (SHR) and adrenocorticotropic hormone (ACTH)-induced hypertensive rat, respectively.
Sixteen-week-old male Wistar-Kyoto (WKY) and age-matched spontaneously hypertensive rats (SHR) were used.
Keywords: Comparison of global gene expression in resistance arteries of normotensive and genetically hypertensive rats and ACTH-treated rats.
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| Overall design |
There were 3 experimental groups: Group 1: 16-week male Wistar-Kyoto rats; Group 2: 16-week male Wistar-Kyoto rats treated with ACTH (0.1mg/kg/day) subcutaneously, for 4 weeks prior to sampling (i.e. during weeks 12-16 of life) ; Group3: 16-week male SHR (spontaneously hypertensive) rats. There were 3 replicate hybridizations in each experimental group. Due to the low yield of total RNA obtained from the arterial sections, each replicate was composed of RNA pooled from 2-3 different rats.
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| Contributor(s) |
Hill CE, Grayson TH |
| Citation(s) |
17986358 |
| |
| Submission date |
Jun 07, 2007 |
| Last update date |
Mar 03, 2017 |
| Contact name |
Stephen John Ohms |
| E-mail(s) |
Stephen.Ohms@anu.edu.au
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| Phone |
61-2-6125-2522
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| Organization name |
Australian National University
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| Department |
John Curtin School of Medical Research
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| Lab |
ACRF Biomolecular Resource Facility
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| Street address |
Garran Road
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| City |
Canberra |
| State/province |
Australian Capital Territory |
| ZIP/Postal code |
GPO Box 334, Canberra City |
| Country |
Australia |
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| Platforms (1) |
| GPL341 |
[RAE230A] Affymetrix Rat Expression 230A Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA100879 |
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