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Series GSE8043 Query DataSets for GSE8043
Status Public on Feb 28, 2008
Title FLT3 gene-expression signature in normal karyotype AML
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Acute myeloid leukemia with normal karyotype (NK-AML) represents a cytogenetic grouping with intermediate prognosis but substantial molecular and clinical heterogeneity. Within this subgroup, presence of FLT3 (FMS-like tyrosine kinase 3) internal tandem duplication (ITD) mutation predicts less favorable outcome. The goal of our study was to discover gene-expression patterns correlated with FLT3-ITD mutation, and to evaluate the utility of a FLT3 signature for prognostication.
The dataset comprises gene-expression profiles of 137 normal karyotype acute myeloid leukemia (NK-AML) specimens carried out using Stanford cDNA microarrays, to accompany the study of L Bullinger et al. For each array, Channel 2 represents Cy5-labeled NK-AML RNA, and Channel 1 Cy3-labeled universal reference RNA.
Keywords: Logical Set
 
Overall design DNA microarrays were used to profile gene expression in a training set of 65 NK-AML cases. Supervised analysis was applied to build a gene expression-based predictor of FLT3-ITD mutation status. The predictor was then evaluated by classifying expression profiles from an independent test set of 72 NK-AML cases.
 
Contributor(s) Bullinger L
Citation(s) 18309032
Submission date Jun 06, 2007
Last update date Mar 17, 2012
Contact name Jonathan Pollack
E-mail(s) pollack1@stanford.edu
Phone 650-736-1987
Organization name Stanford University
Department Pathology
Lab Pollack Lab
Street address 269 Campus Drive, CCSR3245A
City Stanford
State/province CA
ZIP/Postal code 94305-5176
Country USA
 
Platforms (8)
GPL3320 SHCZ
GPL3334 SHCO
GPL3355 SHDJ
Samples (137)
GSM198459 99KM8, 46,XY
GSM198460 98PB345, 46,XX
GSM198461 99PB126, 46,XY
Relations
BioProject PRJNA100863

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Supplementary data files not provided
Processed data included within Sample table

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