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Series GSE80396 Query DataSets for GSE80396
Status Public on Aug 17, 2016
Title Genome-wide alterations in DNA methylation identify Leprel2, Foxf1, Mmp25, Igfbp6 and Peg12 as disease-associated genes in murine Achilles tendinopathy 
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary We partnered with Zymo Research, Inc. to detect genome-wide methylation changes associated with the initiation and progression of tendinopathy induced by TGF-β1 in a murine Achilles tendon model. All genes identified as significantly differentially methylated were present in only one experimental group showing that both the gene identified and its modified methylation status are dependent on the injury time-course and activity level. In summary, we identified five genes (Leprel2, Foxf1, Mmp25, Igfbp6 and Peg12) which demonstrate significant relevance to tendon biology with three exhibiting functions in collagen fibril organization. We postulate that a study of the molecular genomics of these genes in animal and human tendon could further delineate the pathogenesis of this multifactorial disease.
Overall design Chronic Achilles tendinopathy was induced in WT C57/Bl6 male mice via two injections of TGF-β1 directly into the body of the tendon. Methylome analysis was conducted on the tendon DNA for the acute (3 day) response as well as the chronic response (14 and 28 days) under normal cage activity or with treadmill running and compared to un-injured mice.
Contributor(s) Trella KJ, Li J, Stylianou E, Wang VM, Frank JM, Galante J, Sandy JD, Plaas A, Wysocki R
Citation(s) 27517731
Submission date Apr 18, 2016
Last update date May 15, 2019
Contact name Katie J Trella
Organization name Rush University Medical Center
Department Orthopedics
Lab Sports Medicine
Street address 1611 W. Harrison St, Suite 401A
City Chicago
State/province IL
ZIP/Postal code 60612
Country USA
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (6)
GSM2125941 Zr1087_1
GSM2125942 Zr1087_2
GSM2125943 Zr1087_3
BioProject PRJNA318791
SRA SRP073454

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Supplementary file Size Download File type/resource
GSE80396_RAW.tar 482.8 Mb (http)(custom) TAR (of BB, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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