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| Status |
Public on Apr 18, 2016 |
| Title |
Spheroid growth in ovarian cancer promotes gene expression involved in epithelial-mesenchymal transition, stem cell promotion, and multiple stress responses |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In this study, we examine gene expression changes through microarray analysis in spheroid versus monolayer ovarian cancer cells treated with TGFβ to induce EMT. Interestingly, EMT, stress response, and stem cell differentiation pathways were all significantly affected by 3D growth. These findings support the hypothesis that three dimensional ovarian cell culturing is physiologically different from its monolayer counterpart.
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| Overall design |
HEY Ovarian Cancer cells were treated with TGFβ and either cultured as a monolayer or using the hanging drop method to create spheroids. Microarray analysis was conducted on spheroid (3D) versus monolayer cells (2D).
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| Contributor(s) |
Paullin T, Powell C, Menzie C, Hill R, Cheng F, Martyniuk CJ, Westerheide SD |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 18, 2016 |
| Last update date |
Nov 13, 2022 |
| Contact name |
Christopher Martyniuk |
| E-mail(s) |
cmartyn@ufl.edu
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| Phone |
3523173905
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| Organization name |
University of Florida
|
| Street address |
8891 SW 79th Avenue
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| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32608 |
| Country |
USA |
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| Platforms (1) |
| GPL13667 |
[HG-U219] Affymetrix Human Genome U219 Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA318768 |
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