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Series GSE80354 Query DataSets for GSE80354
Status Public on Dec 15, 2016
Title Gata6 promotes hair follicle matrix progenitor cell renewal by genome maintenance via Edaradd/NF-κB
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Cell proliferation is essential to rapid tissue growth and repair, but is inherently associated with considerable genome damage that cells must efficiently prevent or fix to prevent cell cycle arrest. Here, we implicate the transcription factor Gata6 in regulation of adult mouse hair follicle regeneration where it controls the renewal of the rapidly proliferating epithelial (matrix) progenitors and hence the extent of production of terminally differentiated lineages. We find that Gata6 protects against DNA damage associated with proliferation, thus preventing cell cycle arrest and apoptosis. Furthermore, we show that Gata6 stimulates the Eddarad/NF-kB pathway, important for DNA-damage repair and stress response in general, and for hair follicle growth in particular. Finally, we find Edaradd essential, downstream of Gata6 for cell survival and proliferation. Our data add to recent evidence in embryonic stem and neural progenitor cells, suggesting a model whereby developmentally regulated transcription factors protect from DNA damage associated with proliferation occurring at key stages of rapid tissue growth. Our data may aid in understanding why Gata6 is a frequent target of amplification in cancers.
Overall design Gene expression profiling by mRNA-seq to identify differentially expressed genes in wild type (WT) and Gata6 induced knockout (iKO) mouse epidermal keratinocytes
Contributor(s) Wang AB, Zhang YV, Tumbar T
Citation(s) 27908934
Submission date Apr 15, 2016
Last update date May 15, 2019
Contact name Alex Wang
Organization name Cornell University
Department Molecular Biology and Genetics
Street address 260 Biotechnology Bldg
City Ithaca
State/province NY
ZIP/Postal code 14853
Country USA
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (6)
GSM2125013 Gata6 WT2
GSM2125014 Gata6 WT3
GSM2125015 Gata6 WT4
BioProject PRJNA318648
SRA SRP073383

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Supplementary file Size Download File type/resource
GSE80354_Gata6_DEseq2_res.csv.gz 621.8 Kb (ftp)(http) CSV
GSE80354_Gata6_raw_counts.txt.gz 253.5 Kb (ftp)(http) TXT
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